Volume 9, Issue 1 (2023)
Pharm Biomed Res 2023, 9(1): 53-62 |
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Yakubu J, Amos C, Medugu A N, Dawa S I, Abdulrahman F I, Sodipo O A et al . Bioassay-guided Antidiabetic Study of Chromatographic Fractions of Boswellia Dalzielii Hutch. Leaf Extract. Pharm Biomed Res 2023; 9 (1) :53-62
URL: http://pbr.mazums.ac.ir/article-1-495-en.html
URL: http://pbr.mazums.ac.ir/article-1-495-en.html
James Yakubu * 
1, Charity Amos2 , Asinamai Ndai Medugu3 , Samuel Ibrahim Dawa4 , Fanna Inna Abdulrahman1 , Olufunke Adebola Sodipo5 , Bitrus Wampana6
1, Charity Amos2 , Asinamai Ndai Medugu3 , Samuel Ibrahim Dawa4 , Fanna Inna Abdulrahman1 , Olufunke Adebola Sodipo5 , Bitrus Wampana6
1- Department of Pure and Applied Chemistry, Faculty of Science, University of Maiduguri, PMB, Maiduguri, Borno State, Nigeria
2- Department of Pharmacology and Toxicology, Faculty of Pharmacy, University of Maiduguri, Borno State, Nigeria
3- Department of Pharmaceutical Outcomes and Policy, College of Pharmacy, University of Florida, Florida, USA.
4- Department of Pure and Applied Chemistry, Faculty of Science, University of Maiduguri, Maiduguri,Nigeria.
5- Department of Clinical Pharmacology and Therapeutics, College of Medical Science, University of Maiduguri, Maiduguri, Borno State, Nigeria
6- Department of Pharmacology, Faculty of Veterinary Medicine, University of Maiduguri, Nigeria.
2- Department of Pharmacology and Toxicology, Faculty of Pharmacy, University of Maiduguri, Borno State, Nigeria
3- Department of Pharmaceutical Outcomes and Policy, College of Pharmacy, University of Florida, Florida, USA.
4- Department of Pure and Applied Chemistry, Faculty of Science, University of Maiduguri, Maiduguri,Nigeria.
5- Department of Clinical Pharmacology and Therapeutics, College of Medical Science, University of Maiduguri, Maiduguri, Borno State, Nigeria
6- Department of Pharmacology, Faculty of Veterinary Medicine, University of Maiduguri, Nigeria.
Abstract: (1437 Views)
Background: Boswellia dalzielli Hutch. (Burseraceae) is a medicinal plant, which is used locally by the local dwellers for the management and treatment of microbial-related diseases, neurological conditions, stomach spasms, diabetes, etc.
Objectives: This study aimed at isolating a phytochemical of anti-diabetic potentials from the leaf of Boswellia dalzielii in alloxan-induced diabetic rats.
Methods: The n-butanol fraction of the leaf of B. dalzielii was fractionated using column chromatography. Fractions obtained were screened phytochemically and by antidiabetic study.
Results: Encoded column fraction B4 (150 mg/kg) produced a maximum reduction (72.45%) in fasting blood glucose (FBG) of animals after 7 hours, which was significantly (P<0.05) different from the controls (alloxan-induced diabetic rats) and was better than glibenclamide (52.67%). The re-column fractions obtained from fraction B4 were pooled based on similar Rf values and encoded B41-B48, and subjected to further antidiabetic evaluation on alloxan-induced mice. Eight sub-fraction with doses of 50 mg/kg each were administered to all the groups. Fraction B44 had the highest reduction of FBG by 65.63%, whose effect was significantly higher than the non-treated diabetic mice (negative control) and glibenclamide (52.68%) at 2.0 mg. Further purification of sub-fraction B44 with Sephadex LH-20 yielded encoded fractions A, B, and C. Isolate C showed the highest inhibition of glycemia (22.85%) when the dose of 10 mg/kg was administered (p.o).
Conclusion: The antidiabetic effect of the plant in laboratory animals (rats and mice) may be due to the presence of the isolated phenolic compounds.
Objectives: This study aimed at isolating a phytochemical of anti-diabetic potentials from the leaf of Boswellia dalzielii in alloxan-induced diabetic rats.
Methods: The n-butanol fraction of the leaf of B. dalzielii was fractionated using column chromatography. Fractions obtained were screened phytochemically and by antidiabetic study.
Results: Encoded column fraction B4 (150 mg/kg) produced a maximum reduction (72.45%) in fasting blood glucose (FBG) of animals after 7 hours, which was significantly (P<0.05) different from the controls (alloxan-induced diabetic rats) and was better than glibenclamide (52.67%). The re-column fractions obtained from fraction B4 were pooled based on similar Rf values and encoded B41-B48, and subjected to further antidiabetic evaluation on alloxan-induced mice. Eight sub-fraction with doses of 50 mg/kg each were administered to all the groups. Fraction B44 had the highest reduction of FBG by 65.63%, whose effect was significantly higher than the non-treated diabetic mice (negative control) and glibenclamide (52.68%) at 2.0 mg. Further purification of sub-fraction B44 with Sephadex LH-20 yielded encoded fractions A, B, and C. Isolate C showed the highest inhibition of glycemia (22.85%) when the dose of 10 mg/kg was administered (p.o).
Conclusion: The antidiabetic effect of the plant in laboratory animals (rats and mice) may be due to the presence of the isolated phenolic compounds.
Type of Study: Original Research |
Subject:
Ehtnopharmacology
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