Volume 12, Issue 3 (2026)
Pharm Biomed Res 2026, 12(3): 0-0 |
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Tahir A, Sani Muhammad A, Makeri Ayuba G, Abubakar Aminu A, Samaila Lassi A, Abdullahi Kobi K, et al . Phytochemical Composition and Broad-Spectrum Antimicrobial Activity of Guiera senegalensis Methanolic Leaf Extract Against Clinical Isolates. Pharm Biomed Res 2026; 12 (3)
URL: http://pbr.mazums.ac.ir/article-1-706-en.html
URL: http://pbr.mazums.ac.ir/article-1-706-en.html
Albashir Tahir *1 
, Aishatu Sani Muhammad1 , Gideon Makeri Ayuba1 , Al-ameen Abubakar Aminu1 , Aminu Samaila Lassi1 , Khadija Abdullahi Kobi2 , Nazif Ayuba1 , Saddam Ahmad1
, Aishatu Sani Muhammad1 , Gideon Makeri Ayuba1 , Al-ameen Abubakar Aminu1 , Aminu Samaila Lassi1 , Khadija Abdullahi Kobi2 , Nazif Ayuba1 , Saddam Ahmad1
1- Department of Pharmacology, Faculty of Basic Medical Sciences, Sa'adu Zungur University, Bauchi State, Nigeria
2- Department of Nursing, Faculty of Allied Health Sciences, Abubakar Tafawa Balewa University, Bauchi State, Nigeria
2- Department of Nursing, Faculty of Allied Health Sciences, Abubakar Tafawa Balewa University, Bauchi State, Nigeria
Abstract: (8 Views)
Background: The growing threat of antimicrobial resistance has intensified the search for alternative therapies from natural sources. Guiera senegalensis, a medicinal shrub widely used in African traditional medicine, has shown promising antimicrobial properties attributed to its rich phytochemical content. This study evaluated the phytochemical composition, and the in vitro antibacterial and antifungal activities of methanolic leaf extract of Guiera senegalensis against some selected clinical isolates.
Methods: Fresh leaves of G. senegalensis were collected, air-dried, and extracted using 70% methanol. Phytochemical screening was conducted using standard qualitative techniques. Antibacterial and antifungal activities were assessed using agar well and disk diffusion methods. Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal/Fungicidal Concentration (MBC/MFC) were determined via serial dilution.
Results: The extract contained flavonoids, tannins, saponins, steroids, terpenoids, and alkaloids. Antimicrobial activity was concentration-dependent, with E. coli and S. aureus showing the highest bacterial susceptibility (zones of inhibition up to 29 mm) and F. oxysporum exhibiting strong antifungal sensitivity (25 mm at 500 mg/mL). MIC values ranged from 31.25 to 125 mg/mL for bacteria and fungi, while MBC/MFC values ranged from 62.5 to 250 mg/mL.
Conclusion: The methanolic extract of Guiera senegalensis demonstrated broad-spectrum antibacterial and antifungal activity, particularly against Gram-positive bacteria and filamentous fungi.
Methods: Fresh leaves of G. senegalensis were collected, air-dried, and extracted using 70% methanol. Phytochemical screening was conducted using standard qualitative techniques. Antibacterial and antifungal activities were assessed using agar well and disk diffusion methods. Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal/Fungicidal Concentration (MBC/MFC) were determined via serial dilution.
Results: The extract contained flavonoids, tannins, saponins, steroids, terpenoids, and alkaloids. Antimicrobial activity was concentration-dependent, with E. coli and S. aureus showing the highest bacterial susceptibility (zones of inhibition up to 29 mm) and F. oxysporum exhibiting strong antifungal sensitivity (25 mm at 500 mg/mL). MIC values ranged from 31.25 to 125 mg/mL for bacteria and fungi, while MBC/MFC values ranged from 62.5 to 250 mg/mL.
Conclusion: The methanolic extract of Guiera senegalensis demonstrated broad-spectrum antibacterial and antifungal activity, particularly against Gram-positive bacteria and filamentous fungi.
Type of Study: Original Research |
Subject:
Ehtnopharmacology
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