Volume 11, Issue 3 (2025)
Pharm Biomed Res 2025, 11(3): 201-214 |
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Osarieme Imade R, Ayinde B A, Iyawe A O, Ogbemudia C O. Antifibroid and High-performance Liquid Chromatography Analysis of Azadirachta indica (Meliaceae) Leaves. Pharm Biomed Res 2025; 11 (3) :201-214
URL: http://pbr.mazums.ac.ir/article-1-680-en.html
URL: http://pbr.mazums.ac.ir/article-1-680-en.html
Rose Osarieme Imade *1 
, Buniyamin Adesina Ayinde1 , Andrex Osatohanmwen Iyawe1 , Charles Osemwegie Ogbemudia1
, Buniyamin Adesina Ayinde1 , Andrex Osatohanmwen Iyawe1 , Charles Osemwegie Ogbemudia1
1- Department of Pharmacognosy, Faculty of Pharmacy, University of Benin, Benin, Nigeria.
Abstract: (604 Views)
Background: Globally, women’s reproductive health is endangered due to fibroids, benign tumors that develop from the uterine smooth muscle cells. Women are increasingly turning to herbal remedies to treat uterine fibroids due to the disadvantages of conventional therapies. Among these plants, the leaves of Azadirachta indica are particularly noteworthy.
Objectives: This study aimed to evaluate the antifibroid potential of the ethanol leaf extract of A. indica (Meliaceae) in Wistar rats.
Methods: Rats were administered monosodium glutamate (MSG) daily for 30 days to induce the formation of spindle-shaped fibers characteristic of fibroids. The extract was administered in graded doses to a second group as a prophylactic measure concurrently with MSG (preventive approach). In the curative approach, MSG was administered for 30 days to induce fibroid cell formation, following which the extract was administered. Total cholesterol, protein, estrogen, and progesterone levels were measured using biochemical analysis of blood serum. Uterine histopathological examination was performed. High-performance liquid chromatography (HPLC) was employed to identify the bioactive components of the plant.
Results: MSG elevated serum cholesterol (P≤0.05), progesterone (P≤0.001), and estradiol levels in rats. Extract administration inhibited the rise in these parameters, both in the preventive and curative experiments. Histopathology assessment of the rat uterus treated with the extract revealed a reduction in fibroid cells at the 80 mg/kg dose. HPLC analysis identified β-caryophyllin, azadirachnol, azadirachta-A, and quercetin as the major phenolic compounds in the extract.
Conclusion: These findings demonstrate the antifibroid potential of A. indica leaves which merits further research.
Objectives: This study aimed to evaluate the antifibroid potential of the ethanol leaf extract of A. indica (Meliaceae) in Wistar rats.
Methods: Rats were administered monosodium glutamate (MSG) daily for 30 days to induce the formation of spindle-shaped fibers characteristic of fibroids. The extract was administered in graded doses to a second group as a prophylactic measure concurrently with MSG (preventive approach). In the curative approach, MSG was administered for 30 days to induce fibroid cell formation, following which the extract was administered. Total cholesterol, protein, estrogen, and progesterone levels were measured using biochemical analysis of blood serum. Uterine histopathological examination was performed. High-performance liquid chromatography (HPLC) was employed to identify the bioactive components of the plant.
Results: MSG elevated serum cholesterol (P≤0.05), progesterone (P≤0.001), and estradiol levels in rats. Extract administration inhibited the rise in these parameters, both in the preventive and curative experiments. Histopathology assessment of the rat uterus treated with the extract revealed a reduction in fibroid cells at the 80 mg/kg dose. HPLC analysis identified β-caryophyllin, azadirachnol, azadirachta-A, and quercetin as the major phenolic compounds in the extract.
Conclusion: These findings demonstrate the antifibroid potential of A. indica leaves which merits further research.
Keywords: Azadirachta indica, Fibroid, High-performance liquid chromatography (HPLC), Histology, Hormones
Type of Study: Original Research |
Subject:
Pharmacognosy
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