Volume 10, Issue 3 (2024)
Pharm Biomed Res 2024, 10(3): 257-268 |
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Osarieme Imade R, Ayinde B A, Onyebuchi C E, Asoya E V. Antifibroid Evaluation of Tetrapleura tetraptera Taubert (Fabaceae) Stem Bark Ethanol Extract and HPLC Analysis of Its Polyphenolic Compounds. Pharm Biomed Res 2024; 10 (3) :257-268
URL: http://pbr.mazums.ac.ir/article-1-624-en.html
URL: http://pbr.mazums.ac.ir/article-1-624-en.html
Rose Osarieme Imade *1 
, Buniyamin Adesina Ayinde1 , Chinaza Esther Onyebuchi1 , Ekene Victor Asoya2
, Buniyamin Adesina Ayinde1 , Chinaza Esther Onyebuchi1 , Ekene Victor Asoya2
1- Department of Pharmacognosy, Faculty of Pharmacy, University of Benin, Benin, Nigeria.
2- Department of Medical Biochemistry, School of Basic Medical Science, University of Benin, Benin, Nigeria.
2- Department of Medical Biochemistry, School of Basic Medical Science, University of Benin, Benin, Nigeria.
Abstract: (1173 Views)
Background: Uterine fibroids are the most frequently diagnosed tumors of the female reproductive system and the primary reason for hysterectomy. Tetrapleura tetraptera stem bark is traditionally used to treat this condition.
Objectives: To assess the antifibroid activity of the ethanol stem bark extract of T. tetraptera in Sprague-Dawley rats.
Methods: An acute toxicity test was performed to determine the extract’s safety level. Antifibroid activity was evaluated with monosodium glutamate (MSG) administration to rats daily for 30 days and then with concurrent graded doses of the extract (preventive method). A second group of rats were given MSG for 30 days to induce fibroid formation, after which the extract was administered in graded doses for another 30 days (curative method). Biochemical analysis of blood serum was done, and total cholesterol, protein, and estradiol levels were assessed. Histopathological analysis of the uterus was carried out. Major bioactive polyphenolic constituents were identified by high-performance liquid chromatography (HPLC).
Results: The extract exhibited no mortality even at 5000 mg/kg. MSG significantly elevated (P≤0.05) serum cholesterol and estradiol levels in the rats; extract administration inhibited the rise in these parameters, both in the preventive and curative experiments. Histopathology assessment of rat uterus given the extract revealed a reduction in fibroid cells induced by MSG. HPLC analysis identified umbelliferone, piperazine, aridanin, and naringenin as the major phenolic compounds in the extract.
Conclusion: This research’s results support the plant’s antifibroid potential.
Objectives: To assess the antifibroid activity of the ethanol stem bark extract of T. tetraptera in Sprague-Dawley rats.
Methods: An acute toxicity test was performed to determine the extract’s safety level. Antifibroid activity was evaluated with monosodium glutamate (MSG) administration to rats daily for 30 days and then with concurrent graded doses of the extract (preventive method). A second group of rats were given MSG for 30 days to induce fibroid formation, after which the extract was administered in graded doses for another 30 days (curative method). Biochemical analysis of blood serum was done, and total cholesterol, protein, and estradiol levels were assessed. Histopathological analysis of the uterus was carried out. Major bioactive polyphenolic constituents were identified by high-performance liquid chromatography (HPLC).
Results: The extract exhibited no mortality even at 5000 mg/kg. MSG significantly elevated (P≤0.05) serum cholesterol and estradiol levels in the rats; extract administration inhibited the rise in these parameters, both in the preventive and curative experiments. Histopathology assessment of rat uterus given the extract revealed a reduction in fibroid cells induced by MSG. HPLC analysis identified umbelliferone, piperazine, aridanin, and naringenin as the major phenolic compounds in the extract.
Conclusion: This research’s results support the plant’s antifibroid potential.
Keywords: Tetrapleura tetraptera, Fibroid, High-performance liquid chromatography (HPLC), Biochemical, Histopathology
Type of Study: Original Research |
Subject:
Pharmacognosy
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