Volume 10, Issue 4 (2024)
Pharm Biomed Res 2024, 10(4): 319-330 |
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Awatef E, Chaabane M, Ghorbel I, Jebahi S, Marrekchi R, Boudawara T et al . Effects of Barium Chloride on Metallothioneins Induction and Biochemical and Histomorphological Changes in the Kidney of Adult Female Rats. Pharm Biomed Res 2024; 10 (4) :319-330
URL: http://pbr.mazums.ac.ir/article-1-604-en.html
URL: http://pbr.mazums.ac.ir/article-1-604-en.html
Elwej Awatef *1 
, Mariem Chaabane1 , Imen Ghorbel1 , Samira Jebahi2 , Rim Marrekchi3 , Tahia Boudawara1 , Najiba Zeghal4
, Mariem Chaabane1 , Imen Ghorbel1 , Samira Jebahi2 , Rim Marrekchi3 , Tahia Boudawara1 , Najiba Zeghal4
1- Animal Physiology Laborator, Sfax Faculty of Sciences, Sfax University, Sfax, Tunisia.
2- Biochemistry Laboratory, CHU Hedi Chaker, University of Sfax, Sfax, Tunisia.
3- Anatomopathology Laboratory, CHU Habib Bourguiba, Sfax University, Sfax, Tunisia.
4- Animal Physiology Laboratory. Sfax Faculty of Sciences.BP1171, 3000 Sfax. Sfax University, Tunisia
2- Biochemistry Laboratory, CHU Hedi Chaker, University of Sfax, Sfax, Tunisia.
3- Anatomopathology Laboratory, CHU Habib Bourguiba, Sfax University, Sfax, Tunisia.
4- Animal Physiology Laboratory. Sfax Faculty of Sciences.BP1171, 3000 Sfax. Sfax University, Tunisia
Abstract: (906 Views)
Background: Heavy metal exposure usually causes harmful effects on the environment and human health.
Objectives: This study investigates the toxic effects of barium chloride (BaCl2) administered at graded doses to adult rats on kidney oxidative stress biomarkers and histological changes.
Methods: Animals were divided into four groups with six animals in each group. The control group did not receive any treatment, while groups 2, 3, and 4 were exposed to barium at doses of 67, 150, and 300 parts per million for 3 weeks. After recording 24 h urine volumes, samples were collected and stored at -20 ˚C for further analysis. At the end of the experimental period, plasma and kidney tissue were collected for biochemical and histological analysis.
Results: The treatment of barium can induce nephrotoxicity as evidenced by a significant increase of malondialdehyde (MDA), hydrogen peroxide (H2O2), and advanced oxidation protein product (AOPP). A significant increase in kidney levels of total metallothioneins (MTs) was also observed. Superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) activities, reduced glutathione, non-protein thiol, and vitamin C levels showed a significant decline. Plasma creatinine and urea levels showed a significant increase associated with a decrease in uric acid levels. Meanwhile, nephron-biochemical parameters were substantiated by histological examination, revealing disorganized kidney architecture.
Conclusion: The results highlight significant health concerns regarding environmental barium exposure.
Objectives: This study investigates the toxic effects of barium chloride (BaCl2) administered at graded doses to adult rats on kidney oxidative stress biomarkers and histological changes.
Methods: Animals were divided into four groups with six animals in each group. The control group did not receive any treatment, while groups 2, 3, and 4 were exposed to barium at doses of 67, 150, and 300 parts per million for 3 weeks. After recording 24 h urine volumes, samples were collected and stored at -20 ˚C for further analysis. At the end of the experimental period, plasma and kidney tissue were collected for biochemical and histological analysis.
Results: The treatment of barium can induce nephrotoxicity as evidenced by a significant increase of malondialdehyde (MDA), hydrogen peroxide (H2O2), and advanced oxidation protein product (AOPP). A significant increase in kidney levels of total metallothioneins (MTs) was also observed. Superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) activities, reduced glutathione, non-protein thiol, and vitamin C levels showed a significant decline. Plasma creatinine and urea levels showed a significant increase associated with a decrease in uric acid levels. Meanwhile, nephron-biochemical parameters were substantiated by histological examination, revealing disorganized kidney architecture.
Conclusion: The results highlight significant health concerns regarding environmental barium exposure.
Type of Study: Original Research |
Subject:
Toxicology
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