Volume 11, Issue 3 (2025)
Pharm Biomed Res 2025, 11(3): 0-0 |
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Mukanganyama S, Mozirandi W, Fru C G. Inhibition of biofilms by extracts from Vernonia adoensis in Pseudomonas aeruginosa and Staphylococcus aureus. Pharm Biomed Res 2025; 11 (3)
URL: http://pbr.mazums.ac.ir/article-1-551-en.html
URL: http://pbr.mazums.ac.ir/article-1-551-en.html
1- University of Zimbabwe
2- University of Buea
2- University of Buea
Abstract: (22 Views)
Background: Bacteria that form biofilms have become a leading cause of increased morbidity and motility in healthcare settings, as they are responsible for over 65% of nosocomial infections. Biofilms are a major cause of drug resistance in bacteria. Pseudomonas aeruginosa and Staphylococcus aureus are opportunist pathogens that cause numerous infections and are known for their ability to produce biofilms. Vernonia adoensis is an African ethnomedicinal plant commonly used for the treatment of various disease conditions and has been shown to have antibacterial activity.
Objectives: The aim of this study was to investigate the effect of extracts from V. adoensis on the biofilms formed by P. aeruginosa and S. aureus and determine the phytochemicals present in the extract.
Methods: The effect of the extract on the biofilms of P. aeruginosa and S. aureus was determined on 96-microwell plates using crystal violet. Phytochemical constituents of the extract were determined using Ultra-performance liquid chromatography coupled to mass spectroscopy (UPLC-MS).
Results: The extract disrupted the biofilms of the bacteria. At 100 µg/ml, the extract inhibited the formation of biofilms of P. aeruginosa and S. aureus by 93% and 17%, respectively. V. adoensis was more potent in decreasing the attachment efficiency of the biofilm of P. aeruginosa as it caused the detachment of 84% of the biofilm in the presence of SDS but only 17% of the biofilm of S. aureus. Five compounds (kaempferol, quinic acid, caffeic acid, rhamnetin I, and luteolin were identified by UPLC-MS analysis. Some of these compounds have been shown to have some antimicrobial activity.
Conclusions: It can be concluded that V. adoensis contains bioactive components that may be exploited as sources of lead compounds for the development of new antimicrobial agents that have antibiofilm activity.
Objectives: The aim of this study was to investigate the effect of extracts from V. adoensis on the biofilms formed by P. aeruginosa and S. aureus and determine the phytochemicals present in the extract.
Methods: The effect of the extract on the biofilms of P. aeruginosa and S. aureus was determined on 96-microwell plates using crystal violet. Phytochemical constituents of the extract were determined using Ultra-performance liquid chromatography coupled to mass spectroscopy (UPLC-MS).
Results: The extract disrupted the biofilms of the bacteria. At 100 µg/ml, the extract inhibited the formation of biofilms of P. aeruginosa and S. aureus by 93% and 17%, respectively. V. adoensis was more potent in decreasing the attachment efficiency of the biofilm of P. aeruginosa as it caused the detachment of 84% of the biofilm in the presence of SDS but only 17% of the biofilm of S. aureus. Five compounds (kaempferol, quinic acid, caffeic acid, rhamnetin I, and luteolin were identified by UPLC-MS analysis. Some of these compounds have been shown to have some antimicrobial activity.
Conclusions: It can be concluded that V. adoensis contains bioactive components that may be exploited as sources of lead compounds for the development of new antimicrobial agents that have antibiofilm activity.
Type of Study: Original Research |
Subject:
Ehtnopharmacology
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