Volume 10, Issue 1 (2024)
Pharm Biomed Res 2024, 10(1): 23-32 |
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Ukwubile C A, Malgwi T S, Menkiti N D. Examining the Isolation of Bioactive Compounds, Antinociceptive, and Anti-inflammatory Activities of Strychnos Spinosa Lam. (Loganiaceae) Stembark Extract. Pharm Biomed Res 2024; 10 (1) :23-32
URL: http://pbr.mazums.ac.ir/article-1-530-en.html
URL: http://pbr.mazums.ac.ir/article-1-530-en.html
1- Department of Pharmacognosy, Faculty of Pharmacy, University of Maiduguri, Maiduguri, Nigeria.
2- Department of Chemistry, Faculty of Science, Ahmadu Bello University, Zaria, Nigeria.
2- Department of Chemistry, Faculty of Science, Ahmadu Bello University, Zaria, Nigeria.
Abstract: (882 Views)
Background: Strychnos spinosa is a tree whose parts are popularly used in Southeast Nigeria to treat pains, infections, inflammations, hypertension, malaria fever, and ulcers.
Objectives: This study isolates the bioactive compounds in methanol stembark extract and evaluates the antinociceptive and anti-inflammatory potentials of S. spinosa.
Methods: Bioactive compounds were isolated using silica gel column chromatography, and their structures were elucidated using Fourier-transform infrared spectroscopy, gas chromatography/mass spectrometry, liquid chromatography/mass spectrometry, and nuclear magnetic resonance spectroscopy apparatus. The antinociceptive activity was determined using the acetic acid-induced writhing mice model, hot plate method, and tail immersion. In contrast, the anti-inflammatory activity was assessed using carrageenan-induced paw-edema in mice.
Results: The structural elucidation of the major bioactive compounds showed that the compounds are 18-methylnonadecanoate ester, 2-pyridin-3yl-ethanimidamide, 2-ethylformanilide, and acetamide. The extract at the doses of 200, 400, and 800 mg/kg, intraperitoneal significantly decreased (P<0.05) pains in acetic acid-induced writhing in mice, hot plate, and tail immersion assays in a dose-dependent fashion. In the hot plate and tail immersion assays, the extract produced significant pain inhibition of >50% after 120 min at a 400 mg/kg dose. In contrast, the stembark extract of S. spinosa produced the highest inhibition of paw edema formation at a dose of 400 mg/kg in 6 h in the carrageenan-induced paw edema model.
Conclusion: The results affirmed using S. spinosa stembark extract in traditional medicine to treat pain and inflammation. This was possible due to the presence of the isolated compounds.
Objectives: This study isolates the bioactive compounds in methanol stembark extract and evaluates the antinociceptive and anti-inflammatory potentials of S. spinosa.
Methods: Bioactive compounds were isolated using silica gel column chromatography, and their structures were elucidated using Fourier-transform infrared spectroscopy, gas chromatography/mass spectrometry, liquid chromatography/mass spectrometry, and nuclear magnetic resonance spectroscopy apparatus. The antinociceptive activity was determined using the acetic acid-induced writhing mice model, hot plate method, and tail immersion. In contrast, the anti-inflammatory activity was assessed using carrageenan-induced paw-edema in mice.
Results: The structural elucidation of the major bioactive compounds showed that the compounds are 18-methylnonadecanoate ester, 2-pyridin-3yl-ethanimidamide, 2-ethylformanilide, and acetamide. The extract at the doses of 200, 400, and 800 mg/kg, intraperitoneal significantly decreased (P<0.05) pains in acetic acid-induced writhing in mice, hot plate, and tail immersion assays in a dose-dependent fashion. In the hot plate and tail immersion assays, the extract produced significant pain inhibition of >50% after 120 min at a 400 mg/kg dose. In contrast, the stembark extract of S. spinosa produced the highest inhibition of paw edema formation at a dose of 400 mg/kg in 6 h in the carrageenan-induced paw edema model.
Conclusion: The results affirmed using S. spinosa stembark extract in traditional medicine to treat pain and inflammation. This was possible due to the presence of the isolated compounds.
Keywords: Bioactive compounds, Column chromatography, Strychnos spinosa, Antinociceptive, Anti-inflammatory
Type of Study: Original Research |
Subject:
Pharmacognosy
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