Volume 9, Issue 3 (2023)                   Pharm Biomed Res 2023, 9(3): 243-258 | Back to browse issues page


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Faridi F, Bahador N, Shoja S, Abbasi S. Synthesis and Characterization of Ciprofloxacin-loaded Chitosan Nanoparticles for Eradication of Pseudomonas aeroginosa Biofilm. Pharm Biomed Res 2023; 9 (3) :243-258
URL: http://pbr.mazums.ac.ir/article-1-494-en.html
1- Department of Microbiology, Faculty of Science, Agriculture and New Technologies, Shiraz Branch, Islamic Azad University, Shiraz, Iran.
2- Infectious and Tropical Diseases Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
3- Department of Pharmaceutics, Faculty of Pharmacy, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
Abstract:   (367 Views)
Background: Pseudomonas aeruginosa biofilm is one of the problems in antibiotic treatment of infections. Nanomedicines, such as chitosan (CS) can carry multiple drugs and improve the therapeutic effects of antibiotics.
Objectives: This study aimed at the synthesis and characterization of ciprofloxacin-loaded chitosan nanoparticles for eradication of P. aeroginosa biofilm.
Methods: Cipro-CS microparticles were prepared by ionic gelation method and their size, zeta potential, and drug release pattern were determined. MBEC and MBIC of different groups of antibiotics (ciprofloxacin, ciprofloxacin-PAβN, CS ciprofloxacin, and CS ciprofloxacin-PAβN) were performed on biofilm samples of P. aeroginosa. 
Results: Ciprofloxacin loading efficiency was 35.51%, and encapsulation efficiency was 55.06%. Released ciprofloxacin from CS nanoparticles was 80% after 24 hours. Biofilm production was positive in 96.7% of the isolates while 30.1% of the samples had strong biofilm. The best result for MBIC was CS ciprofloxacin, CS ciprofloxacin-PAβN, ciprofloxacin- PAβN, and ciprofloxacin, respectively. For MBEC the result was slightly different and from the best to better CS Ciprofloxacin-PAβN, CS Ciprofloxacin, Ciprofloxacin-PAβN, and ciprofloxacin.
Conclusion: Today, with increasing antibiotic resistance, there are many challenges in treating infections. Due to the role of biofilm in antibiotic resistance, researchers are looking for new antibiotics to treat infections.
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Type of Study: Original Research | Subject: General

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