Volume 6, Issue 4 (2020)                   Pharm Biomed Res 2020, 6(4): 303-312 | Back to browse issues page


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Ghadiri K, Raofie F, Qomi M, Davoodi A. Response Surface Methodology for Optimization of Supercritical Fluid Extraction of Orange Peel Essential Oil. Pharm Biomed Res. 2020; 6 (4) :303-312
URL: http://pbr.mazums.ac.ir/article-1-310-en.html
1- Active Pharmaceutical Research Center (APIRC), Pharmaceutical Science Branch, Islamic Azad University, Tehran, Iran.
2- Department of Chemistry, Faculty of Science, Shahid Beheshti University, Tehran, Iran.
3- Department of Pharmacognosy and Biotechnology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.
Abstract:   (986 Views)
Background: Orange peel essential oils were obtained using supercritical fluid extraction. This method is an important high scaling extraction method used for the extraction of plant and animal extracts.
Objectives: The aim of this study was the optimization of the extraction of the orange peel essential oil.
Methods: The experimental parameters of Supercritical Fluid Extraction (SFE) such as temperature, pressure, and extraction time, and modifier volume were optimized using a central composite design after a 24-1 fractional factorial design. Orange peels were collected from ripe orange and were washed, air dried, and milled. Then, a Suprex MPS/225 system in the SFE mode with a maximal operating pressure of 395 bars was used for essential oil extraction. Moreover, GC-MS and GC-FID were used for the identification and determination of oil compounds, respectively.
Results: Eight compounds have been identified based on their retention indices and mass spectra. According to the results, α-pinene, β-pinene, myrcene, d-limonene, terpinolene, C8-aldehyde, citronellol, and linalool were identified in orange peel essential oil. The optimum SFE conditions were obtained at a pressure of 347.07 atm, temperature of 55° C, extraction time of 30.16 min, and ethanol volume of 147.05 µL. Moreover, extraction yields based on SFE varied in the range of 0.04% to 1.18% (w/w). 
Conclusion: The Results showed supercritical fluid technology as the best alternative technique for the extraction of pure and high-quality essential oil from orange peel. It is a green method and does not have any environmental impact.
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Type of Study: Original Research | Subject: Pharmacognosy

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