Volume 12, Issue 3 (2026)
Pharm Biomed Res 2026, 12(3): 0-0 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Koochaki R, Nabiuni M, Amini E. In vitro and in vivo cytotoxic and anti-cancer effects of Quillaja saponaria bark saponin against ovarian cancer cells. Pharm Biomed Res 2026; 12 (3)
URL: http://pbr.mazums.ac.ir/article-1-755-en.html
URL: http://pbr.mazums.ac.ir/article-1-755-en.html
1- Department of Cell & Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran.
2- Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran.
2- Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran.
Abstract: (7 Views)
Background: Ovarian Cancer metastasis is the leading cause of cancer-related deaths in women worldwide. The effects of Quillaja saponaria bark saponin (QBS) on the viability and epithelial–mesenchymal transition (EMT) potential of human epithelial ovarian SKOV-3 and A2780 cells, and its anti-metastatic and anti-cancer efficacy of QBS in ovarian cancer model of rat have been investigated.
Methods: The cell cytotoxicity of QBS against SKOV3 and A2780 cells evaluated by MTT assay to calculate IC50 values. Cell migration and invasion assessed by scratch assay. To explore metastasis molecular changes, the expression of key epithelial mesenchymal transition (EMT)-related genes, including α5-integrin, MMP2, Zeb1, E-cadherin measured by real-time PCR. For in vivo assay, DMBA-induced ovarian cancer rat model used.
Results: MTT assay demonstrated that QBS didn’t exert significant toxicity on NIH3T3 as normal cells, nevertheless induced cytotoxicity against A2780 and SKOV3 cells in a dose and time-dependent manner, IC50 was determined as 82 and 77 μg/ml for SKOV3 and A2780 cells, respectively. The scratch assay revealed that QBS significantly attenuated cell invasion at suc-IC50 concentrations (20 and 30 µg/ml). Gene expression indicated down-regulation of Zeb1, α5-integrin and MMP2 at IC50 value of both SKOV3 and A2780 cells (p ≤ 0.001). Also, up-regulation of E-cadherin for both cell lines at IC50 value were significant (p ≤ 0.001). In vivo assay exhibited that QBS significantly reduced tumor growth (p≤0.01) and protected rats against DMBA-induced ovarian cancer with an optimal effect at the dose of 16 mg/kg.
Conclusion: Overall, QBS can offer as potent anti-tumoral agent against ovarian cancer cells in vitro and in vivo.
Methods: The cell cytotoxicity of QBS against SKOV3 and A2780 cells evaluated by MTT assay to calculate IC50 values. Cell migration and invasion assessed by scratch assay. To explore metastasis molecular changes, the expression of key epithelial mesenchymal transition (EMT)-related genes, including α5-integrin, MMP2, Zeb1, E-cadherin measured by real-time PCR. For in vivo assay, DMBA-induced ovarian cancer rat model used.
Results: MTT assay demonstrated that QBS didn’t exert significant toxicity on NIH3T3 as normal cells, nevertheless induced cytotoxicity against A2780 and SKOV3 cells in a dose and time-dependent manner, IC50 was determined as 82 and 77 μg/ml for SKOV3 and A2780 cells, respectively. The scratch assay revealed that QBS significantly attenuated cell invasion at suc-IC50 concentrations (20 and 30 µg/ml). Gene expression indicated down-regulation of Zeb1, α5-integrin and MMP2 at IC50 value of both SKOV3 and A2780 cells (p ≤ 0.001). Also, up-regulation of E-cadherin for both cell lines at IC50 value were significant (p ≤ 0.001). In vivo assay exhibited that QBS significantly reduced tumor growth (p≤0.01) and protected rats against DMBA-induced ovarian cancer with an optimal effect at the dose of 16 mg/kg.
Conclusion: Overall, QBS can offer as potent anti-tumoral agent against ovarian cancer cells in vitro and in vivo.
Keywords: Ovarian cancer metastasis, Quillaja saponaria bark saponin, α5-integrin, MMP2, Zeb1, E-cadherin.
Type of Study: Original Research |
Subject:
Cellular and Molecular Biology
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC-By-NC), which permits use, distribution, and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
Contact Information
