Volume 11, Issue 4 (2025)
Pharm Biomed Res 2025, 11(4): 369-384 |
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Adeyemo A G, Hezekiah Awojide S, Ololade Tiamiyu A, Anifowose A J, Adeboye A O, Olumide Fadunmade E, et al . Effect of Storage Period on the Bioactive Composition and Therapeutic Potential of Syzygium aromaticum Essential Oil. Pharm Biomed Res 2025; 11 (4) :369-384
URL: http://pbr.mazums.ac.ir/article-1-712-en.html
URL: http://pbr.mazums.ac.ir/article-1-712-en.html
Abayomi Gideon Adeyemo *1 
, Shola Hezekiah Awojide1 , Alilat Ololade Tiamiyu1 , Adebanjo Jacob Anifowose2 , Adedayo Olubunmi Adeboye3 , Ezekiel Olumide Fadunmade4 , Oluwatumininu Abosede Mutiu5 , Yemisi Elizabeth Asibor1
, Shola Hezekiah Awojide1 , Alilat Ololade Tiamiyu1 , Adebanjo Jacob Anifowose2 , Adedayo Olubunmi Adeboye3 , Ezekiel Olumide Fadunmade4 , Oluwatumininu Abosede Mutiu5 , Yemisi Elizabeth Asibor1
1- Department of Pure and Applied Chemistry, Faculty of Basic and Applied Sciences, Osun State University, Osogbo, Nigeria.
2- Anifowose
3- Department of Food Science and Technology, Faculty of Basic and Applied Sciences, Osun State University, Osogbo, Nigeria.
4- Learning and Development Bureau, Chatham, United Kingdom.
5- Industrial Chemistry Program, Bowen University, Iwo, Nigeria.
2- Anifowose
3- Department of Food Science and Technology, Faculty of Basic and Applied Sciences, Osun State University, Osogbo, Nigeria.
4- Learning and Development Bureau, Chatham, United Kingdom.
5- Industrial Chemistry Program, Bowen University, Iwo, Nigeria.
Abstract: (62 Views)
Background: Essential oil (EO) of Syzygium aromaticum is usually used for medicinal purposes. In most cases, it is kept for a long duration.
Objectives: The work aimed at examining how the duration of storage of the EO affects its medicinal quality of the essential oil.
Methods: The EO of S. aromaticum was extracted by hydrodistillation. The extracted EO was kept in a cupboard in amber bottles at room temperature for 4, 183, and 365 days. Chemical components of the EOs were determined by gas chromatography mass spectroscopy (GC-MS). The antioxidant activity was determined by using the 2,2-diphenyl-1-picryl-hydrazyl (DPPH), 2,2-azobis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS), and ferric reducing antioxidant power (FRAP) assays. Dopamine and butyrylcholinesterase (BCHE) assays were used to assess the anti-hypertensive activity. The anti-diabetic tendencies were evaluated with the α-glucosidase and α-amylase assays. Albumin denaturation and protease were used to estimate the anti-inflammatory activity.
Results: The results indicate that the EO shows a color change from light yellow in 4 and 183 days of storage to dark brown after 365 days. The results of the GC-MS show a higher value of eugenol in the EO stored for 183 and 365 days (93.15% and 93.46%, respectively). It also shows the presence of eugenol acetate (17.05%) in the EO stored for 4 days, but it was absent in 183 and 365 days of storage. The EO stored for 4 days had a higher inhibition of antioxidant, anti-inflammatory, anti-hypertensive, and anti-diabetic parameters tested.
Conclusion: The results indicate that the higher the period of storage of the essential oil, the less medicinal potency it possesses.
Objectives: The work aimed at examining how the duration of storage of the EO affects its medicinal quality of the essential oil.
Methods: The EO of S. aromaticum was extracted by hydrodistillation. The extracted EO was kept in a cupboard in amber bottles at room temperature for 4, 183, and 365 days. Chemical components of the EOs were determined by gas chromatography mass spectroscopy (GC-MS). The antioxidant activity was determined by using the 2,2-diphenyl-1-picryl-hydrazyl (DPPH), 2,2-azobis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS), and ferric reducing antioxidant power (FRAP) assays. Dopamine and butyrylcholinesterase (BCHE) assays were used to assess the anti-hypertensive activity. The anti-diabetic tendencies were evaluated with the α-glucosidase and α-amylase assays. Albumin denaturation and protease were used to estimate the anti-inflammatory activity.
Results: The results indicate that the EO shows a color change from light yellow in 4 and 183 days of storage to dark brown after 365 days. The results of the GC-MS show a higher value of eugenol in the EO stored for 183 and 365 days (93.15% and 93.46%, respectively). It also shows the presence of eugenol acetate (17.05%) in the EO stored for 4 days, but it was absent in 183 and 365 days of storage. The EO stored for 4 days had a higher inhibition of antioxidant, anti-inflammatory, anti-hypertensive, and anti-diabetic parameters tested.
Conclusion: The results indicate that the higher the period of storage of the essential oil, the less medicinal potency it possesses.
Keywords: Syzygium aromaticum, Essential oil, Antioxidant, Anti-inflammatory, Anti-hypertensive, Antidiabetic
Type of Study: Original Research |
Subject:
Natural products
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