Volume 10, Issue 2 (2024)
Pharm Biomed Res 2024, 10(2): 135-146 |
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Stephen H, Bello B. Protective Effect of Lantana camara Methanolic Leaf Extract Against Acetaminophen-induced Liver Injury in Male Albino Rats. Pharm Biomed Res 2024; 10 (2) :135-146
URL: http://pbr.mazums.ac.ir/article-1-596-en.html
URL: http://pbr.mazums.ac.ir/article-1-596-en.html
1- Department of Biochemistry, Faculty of Life Sciences, Modibbo Adama University, Yola, Nigeria.
Abstract: (1274 Views)
Background: Lantana camara is used traditionally against liver ailments, but scientific evidence for its hepatoprotective effects is limited.
Objectives: This study evaluates the hepatoprotective effect of methanolic leaf extract of L. camara against acetaminophen-(APAP) induced hepatotoxicity in a rat model.
Methods: Phytochemical analysis revealed the presence of phenolics (5.03%), alkaloids (4.30%), saponins (6.14%), tannins (3.81%) and flavonoids (3.54%) in the extract. Methanolic leaf extract of L. camara was administered orally to rats at 100, 300 and 500 mg/kg doses for 5 days before APAP challenge at 750 mg/kg. Serum liver enzymes, proteins, bilirubin and histopathology were assessed to determine the extent of liver injury and protection.
Results: APAP administration caused significant (P<0.05) elevation of serum aspartate transaminase (AST) (89.57±6.90 U/L), alanine transaminase (ALT) (35.61±0.81 U/L), alkaline phosphatase (ALP) (124.90±1.94 U/L), and bilirubin levels along with a reduction of total protein (57.77±0.73 g/L) and albumin levels (25.21±1.08 g/L). It also induced severe hepatocellular necrosis and inflammation. Pretreatment with 300 mg/kg extract dose conferred optimal protection by normalizing enzyme activities (AST: 58.61±2.88 U/L, ALT: 23.57±1.71 U/L, ALP: 92.99±9.87 U/L) and liver function biomarkers (total protein: 80.42±1.72 g/L, albumin: 27.79±1.91 g/L). It also preserved near-normal liver tissue architecture with minimal necrosis, indicating its antioxidant and membrane-stabilizing properties.
Conclusion: The findings validate the ethnomedicinal use of L. camara and indicate its potential for development as an affordable therapy for APAP overdose liver damage.
Objectives: This study evaluates the hepatoprotective effect of methanolic leaf extract of L. camara against acetaminophen-(APAP) induced hepatotoxicity in a rat model.
Methods: Phytochemical analysis revealed the presence of phenolics (5.03%), alkaloids (4.30%), saponins (6.14%), tannins (3.81%) and flavonoids (3.54%) in the extract. Methanolic leaf extract of L. camara was administered orally to rats at 100, 300 and 500 mg/kg doses for 5 days before APAP challenge at 750 mg/kg. Serum liver enzymes, proteins, bilirubin and histopathology were assessed to determine the extent of liver injury and protection.
Results: APAP administration caused significant (P<0.05) elevation of serum aspartate transaminase (AST) (89.57±6.90 U/L), alanine transaminase (ALT) (35.61±0.81 U/L), alkaline phosphatase (ALP) (124.90±1.94 U/L), and bilirubin levels along with a reduction of total protein (57.77±0.73 g/L) and albumin levels (25.21±1.08 g/L). It also induced severe hepatocellular necrosis and inflammation. Pretreatment with 300 mg/kg extract dose conferred optimal protection by normalizing enzyme activities (AST: 58.61±2.88 U/L, ALT: 23.57±1.71 U/L, ALP: 92.99±9.87 U/L) and liver function biomarkers (total protein: 80.42±1.72 g/L, albumin: 27.79±1.91 g/L). It also preserved near-normal liver tissue architecture with minimal necrosis, indicating its antioxidant and membrane-stabilizing properties.
Conclusion: The findings validate the ethnomedicinal use of L. camara and indicate its potential for development as an affordable therapy for APAP overdose liver damage.
Type of Study: Original Research |
Subject:
Ehtnopharmacology
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