en رادیو شیمی Radio Chemistry پژوهشي Original Research <p>PR81 is a monoclonal antibody that binds with high affinity to MUC1 that over expressed on breast tumors. PR81 is considered a suitable targeting molecule that was radiolabeled using Cu-64 for positron imaging studies. The monoclonal antibody was conjugated with DOTA moiety and after purification was evaluated for radiochemical purity, immunoreactivity, cell toxicity and structure integrity as well as biodistribution study in normal rats. The radiolabeled antibody prepared with acceptable radiochemical purity (> 93.2 &plusmn; 0.6 %, ITLC; specific activity; 4.6 &micro;Ci/&micro;g), protein structure integration, significant cytotoxicity and significant immunoreactivity retention was assessed by radioimmunoassay (RIA). Animal biodistribution of the ⁶⁴Cu-DOTA-PR81 was consistent with other radiolabeled antibodies. The results showed that ⁶⁴Cu-DOTA-PR81 may be considered for tumor imaging for ultimate diagnosis and follow-up of MUC1 expression in oncology.</p> Copper-64, PR81, MUC1, tumor, biodistribution 31 46 http://pbr.mazums.ac.ir/browse.php?a_code=A-10-57-4&slc_lang=en&sid=1 Behrouz Alirezapour 1003194753284600970 1003194753284600970 No Radiation Application Research School, Nuclear Science and Technology Research Institute (NSTRI), Tehran, Iran Mohammad Javad Rasaee 1003194753284600971 1003194753284600971 No Department of Clinical Biochemistry, School of Medical Sciences, Tarbiat Modares University (TMU), Tehran, Iran Amir Reza Jalilian 1003194753284600972 1003194753284600972 Yes Radiation Application Research School, Nuclear Science and Technology Research Institute (NSTRI), Tehran, Iran Malihe Paknejad 1003194753284600973 1003194753284600973 No Department of Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran