en فارماسیوتیکس Pharmaceutics پژوهشي Original Research <p>In this study, nystatin liposomal formulation was prepared and characterized. The physicochemical properties of formulations including vesicle size, drug entrapment stability and <em>in vitro</em> release were studied. The highest entrapment efficiency of nystatin into liposomes was obtained about 70% when cholesterol (CHO) was added to the formulations prepared with <a name="OLE_LINK20"></a><a name="OLE_LINK19">dipalmitoylphosphatidylcholine</a>. In addition, the drug entrapment efficiency was decreased when distearoylphosphatidylcholine was used&nbsp;&nbsp; but it was improved by addition of CHO and hydration&nbsp; &nbsp;with 9% sucrose solution. Liposomes with uniform size distribution and average size of 100 nm were produced. &nbsp;Long term stability study indicated that the lyophilized liposomal nystatin was physically stable for at least 6 months at 4 &deg;C. <em>In vitro</em> anti-fungal activity of liposomal nystatin was found to be more effective than free nystatin against <em>Candida albicans</em>.</p> Liposome, lyophilization, antifungal activity, nystatin, nanotechnology 44 54 http://pbr.mazums.ac.ir/browse.php?a_code=A-10-62-2&slc_lang=en&sid=1 Ebrahim Saadat 10031947532846003858 10031947532846003858 No Department of Pharmaceutics, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran Rassoul Dinarvand 10031947532846003859 10031947532846003859 No Medical Nanotechnology Research Centre, Tehran University of Medical Sciences, Tehran, Iran Pedram Ebrahimnejad 10031947532846003860 10031947532846003860 Yes Departmentof Pharmaceutics, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran