Pharmaceutical and Biomedical Research
Pharm Biomed Res
Medical Sciences
http://pbr.mazums.ac.ir
1
admin
2423-4486
2423-4494
10.29252/pbr
8888
en
jalali
1394
9
1
gregorian
2015
12
1
1
4
online
1
fulltext
en
Method validation of clonidine hydrochloride in human plasma by LC-MS technique
آنالیز دارو
Drug Analysis
پژوهشي
Original Research
<p>A simple and sensitive high performance liquid chromatography-electrospray ionization mass spectrometry method has been evaluated for the assignment of clonidine hydrochloride in human plasma. The mobile phase composed of acetonitrile–water 60:40 (v/v), and 0.2% formic acid 20 µL of sample was chromatographically analyzed using a repacked ZORBAX-XDB-ODS C<sub>18</sub> column (2.1 mm×30 mm, 3.5 micron). Detection of analytes was achieved by tandem mass spectrometry with electrospray ionization (ESI) interface in positive ion mode was operated under the multiple-reaction monitoring mode (m/z 230.0 → 213). Sample pretreatment involved in a one-step protein precipitation (PPT) with methanol and percholoric acid (HClO<sub>4</sub>) of 0.15 mL plasma. Standard curve was linear (<em>r </em>= 0.998) over the concentration range of 0.01-10.0 ng/ml and showed suitable accuracy and precision. The limit of quantification (LOQ) was 0.01 ng/ml. The method is rapid, simple, very steady and precise for the separation, assignment, evaluation of clonidine healthy in human plasma.</p>
Clonidine hydrochloride, LC-MS, Human plasma
48
58
http://pbr.mazums.ac.ir/browse.php?a_code=A-10-47-6&slc_lang=en&sid=1
Hossein
Danafar
10031947532846001082
10031947532846001082
Yes
Zanjan Pharmaceutical Nanotechnology Research Center, Zanjan University of Medical Sciences, Zanjan, Iran