en طب سنتی Traditional Medicine پژوهشي Original Research <div style="text-align: justify;"><strong>Background</strong>: The global promotion of herbal products is not at par due to a lack of quality control studies. Marham-i-Raal, a pharmacopoeial ointment, treats various types of ulcers and wounds.&nbsp;<br> <strong>Objectives</strong>: The present study evaluates the quality control, antimicrobial, and antioxidant properties of Marham-i-Raal.<br> <strong>Methods</strong>: The quality control parameters, including loss of weight on drying, pH, total ash value, acid-insoluble ash value, heavy metal analysis, microbial load, and high-performance thin-layer chromatography of Marham-i-Raal, were carried out. Moreover, the antimicrobial and antioxidant activities of Marham-i-Raal were also investigated.&nbsp;<br> <strong>Results</strong>: The quality control findings of Marham-i-Raal, including weight loss on drying, pH, total ash value, acid-insoluble ash value, heavy metal analysis, and microbial load, were found within the normal limits. The antimicrobial study of Marham-i-Raal has shown promising effects against gram-positive bacteria and Candida albicans. The antioxidant effects of the test drug were also found to be significant at higher concentrations against 2,2-diphenyl-1-picrylhydrazyl and 2,2&rsquo;-azino-bis-(3-ethylbenzothiazoline-6-sulfonic) acid methods.&nbsp;<br> <strong>Conclusion</strong>: The standards developed for the quality control of Marham-i-Raal may be referred to in the future for further studies. This important Unani ointment may be clinically used to treat non-healing ulcers and wounds.</div> Marham-i-Raal, Unani ointment, Quality control, HPTLC, Antimicrobial, Antioxidant 47 56 http://pbr.mazums.ac.ir/browse.php?a_code=A-10-1228-1&slc_lang=en&sid=1 Athar Parvez Ansari aatharparvez@gmail.com 100319475328460010371 100319475328460010371 Yes Regional Research Institute of Unani Medicine, Chennai (NABH Accredited), New Delhi, India. Mary Shamya Arokiarajan sowmi.ms.92@gmail.com 100319475328460010372 100319475328460010372 No Regional Research Institute of Unani Medicine, Chennai (NABH Accredited), New Delhi, India. Wasim Akram Syed Akbar 100319475328460010373 100319475328460010373 No Regional Research Institute of Unani Medicine, Chennai (NABH Accredited), New Delhi, India. N Zaheer Ahmed 100319475328460010374 100319475328460010374 No Central Council for Research in Unani Medicine, Ministry of Ayush, Government of India, New Delhi, India. Noman Anwar 100319475328460010375 100319475328460010375 No Regional Research Institute of Unani Medicine, Chennai (NABH Accredited), New Delhi, India.