en ترکیبات طبیعی Natural products پژوهشي Original Research <p><em>Ziziphus jujuba Mill </em>(ZJ), which has been extensively used by the Iranian traditional healers, belongs to the <em>Ramnaceae </em>family. This semitropical herb contains large quantities of polyphenols and flavonoids, which in turn reveal antioxidant, antibacterial, free radical scavengering, and several other pharmacological activities. The purpose of the present study was to evaluate the DNA damage prevention potential of hydroalcoholic and polyphenolic extracts of <em>Ziziphus jujuba</em> on HepG2 cells. Throughout the assessment of genoprotective properties, cells were incubated with various concentrations of hydroalcoholic (0.1, 1, 10, and 50 &micro;g/ml) and polyphenolic extracts (0.1 and 1 &micro;g/ml) for a one-hour period, followed by a one-hour incubation period with genotoxic concentration of methyl methanesulfonate (MMS) (10 &micro;M). The comet assay method was applied because of its being attributable to the substantial sensitivity, its inexpensiveness, and its straightforward procedure of use. The tail length, percentage of DNA in tail, and tail moment were measured. Statistical analysis revealed that concentrations of 10 &mu;g/ml for hydroalcoholic extract and 1&mu;g/ml for polyphenolic extract were genoprotective against MMS. Therefore, our results suggest that <em>Ziziphus jujuba</em> at suitable doses can prevent DNA damage.</p> Ziziphus Jujuba, Genoprotective effect, HepG2, Comet Assay. 20 30 http://pbr.mazums.ac.ir/browse.php?a_code=A-10-26-17&slc_lang=en&sid=1 Mahmoud Etebari 10031947532846001106 10031947532846001106 Yes Department of Pharmacology and Toxicology, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran. Behzad Zolfaghari 10031947532846001107 10031947532846001107 No Department of Pharmacognosy, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran Abbas Jafarian-Dehkordi 10031947532846001108 10031947532846001108 No Department of Pharmacology and Toxicology, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran. Afsaneh Mirzaei 10031947532846001109 10031947532846001109 No Department of Pharmacology and Toxicology, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.