en سم شناسی Toxicology پژوهشي Original Research <p>The objective of the present study was to investigate the role of nanocarrier of magnetic isotope of 25-Mg²⁺ (PMC16) in liver toxicity, ATP content and oxidative stress due to malathion (MAL) exposure. PMC16 nanoparticles were administered in different doses (0.05, 0.1 and 0.2 LD₅₀) intravenously (iv) 40 minutes after a single MAL (0.25 LD₅₀= 207 mg/kg) intraperitoneal (ip) injection as a complement to standard therapy of pralidoxime (PAM) and atropine (AT). MgSO₄was used as another supplement for comparison with PMC16. ATP/ADP ratio, antioxidant enzymes including catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD), and oxidative stress biomarker including lipid peroxidation (LPO) were evaluated in liver tissue cells. Results indicated that after MAL administration, ADP/ATP ratio had a significant increase in liver tissues in comparison with control but this ratio was improved using various doses of PMC16. LPO was significantly decreased at all doses of PMC16 and MgSO₄when compared with MAL-exposed group. SOD and CAT activities significantly were increased in MAL-treated group as compared to saline group. SOD was reduced by all doses of PMC16 and CAT activity was decreased in PMC16-0.1 group. These results lead us to conclude that PMC16 and MgSO₄ are so useful for protection against MAL-induced liver toxicity, ATP depletion and oxidative damages.</p> Organophosphate, malathion, magnesium, nanocarrier, adenosine triphosphate, oxidative stress 10 19 http://pbr.mazums.ac.ir/browse.php?a_code=A-10-26-11&slc_lang=en&sid=1 Azam Bakhtiarian 10031947532846001135 10031947532846001135 No Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran Mohammad Abdollahi 10031947532846001136 10031947532846001136 No Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, Iran Seyed Mahdi Rezayat 10031947532846001137 10031947532846001137 No Faculty of Advanced Sciences and Technology in Medicine, Tehran University of Medical Sciences, Tehran, Iran Hamid Reza Mohammadi 10031947532846001138 10031947532846001138 Yes Pharmacutical Science Research Center, Hemoglobinopathy Institute, Mazandaran University of Medical Sciences, Sari, Iran Department of Toxicology and Pharmacology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran