en فارماکولوژی Pharmacology پژوهشي Original Research <strong>Background</strong>: This research aimed to assess the effect of kombucha tea on reducing cholesterol (HCH) uptake and ameliorating biochemical and physiological side effects of high cholesterol diets.&nbsp;<br> <strong>Objectives</strong>: The aim of this research was evaluate the effectiveness of Kombucha tea in reducing cholesterol uptake as well as pathological and immunological side effects &nbsp;from high cholesterol diets in Rabbit.&nbsp;<br> <strong>Methods</strong>: A total of 32 rabbits were assigned into four groups: 1. Control, fed with standard pellets; 2. HCh+St, fed with high cholesterol diet; 3. HCh+Kom, fed with high cholesterol diet and orally received kombucha beverage; and 4. Kom+St, orally received kombucha beverage along with standard diet. Then, the blood Cholesterol (CHO), Triglycerides (TG), Low-density Lipoprotein (LDL), Malondialdehyde (MDA), ferritin, Creatine Phosphokinase (CPK), Interleukin-1 (IL-1), interleukin-6 (IL-6), and liver enzymes (alanine transaminase [ALT], and aspartate transaminase [ AST]), as well as liver histopathological features, were evaluated in all groups.<br> <strong>Results</strong>: CHO and TG showed the highest level in the blood of the HCh+St group, whereas those gavaged with HCh+Kom displayed no change in TG and LDL. Conversely, the HCh+Kom group showed a significant increase in HDL when compared with HCh+St and Kom+St groups. MDA ferritin and CPK in the Kom+St group was significantly higher than the other groups, followed by HCh+Kom. No significant difference was observed in the values of IL-1 and IL-6 as well as ALT and AST between the experimental groups and the control group. Histopathological observations of the liver tissues revealed foam cells with an acidophilic appearance in the HCh+St group and hyperemia in the sinusoid of HCh+Kom and Kom+St groups. Degradation in pancreatic acini was observed in the HCh+St rabbits.&nbsp;<br> <strong>Conclusion</strong>: The data suggest that kombucha consumption is effective in preventing problems caused by high cholesterol diets. Kombucha tea, Hypercholesterolemia, Biochemical side effects, Physiological side effects 123 132 http://pbr.mazums.ac.ir/browse.php?a_code=A-10-700-3&slc_lang=en&sid=1 Monir Doudi monirdoudi@yahoo.com 10031947532846005276 10031947532846005276 No Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran. Zahra Hooshmandi zhoushmandi@yahoo.com 10031947532846005277 10031947532846005277 No Department of Biology, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran. Somayeh Saedi saedi@yahoo.com 10031947532846005278 10031947532846005278 No Department of Biology, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran. Mahbubeh Setorki .setorgi@izehiau.ac.ir 10031947532846005279 10031947532846005279 Yes Department of Biology, Izeh Branch, Islamic Azad University, Izeh, Iran.