Pharmaceutical and Biomedical Research
Pharm Biomed Res
Medical Sciences
http://pbr.mazums.ac.ir
1
admin
2423-4486
2423-4494
10.29252/pbr
8888
en
jalali
1398
8
1
gregorian
2019
11
1
5
4
online
1
fulltext
en
Protective Effect of Curcumin on the Density of Hippocampal Dark Neurons in Mice Model of Aging Induced by D-galactose: A Histopathological Study
عمومی
General
پژوهشي
Original Research
<div style="text-align: justify;"><strong>Background:</strong> Curcumin is the most active ingredient in turmeric root of Curcuma longa of the Zingiberaceae family and has a potent antioxidant activity. This study aimed at investigating the effects of curcumin with various doses on the density of dark neurons in the hippocampus of induced D-galactose aging mice model. This experimental study was conducted on 40 adult male BALB/c mice. <br>
<strong>Materials and Methods:</strong> We randomly divided animals into 5 groups: D-galactose, control, and curcumin 1, curcumin 2, and curcumin 3 groups. D-galactose (300 mg/kg) was intraperitoneally injected into the D-galactose group for 6 weeks. D-galactose and doses of 25, 50, and 100 mg of curcumin were administrated, respectively, to the curcumin groups 1-3 every day for 14 days. After 6 weeks, the mice’s brains were stained with toluidine blue after tissue passage. Then, the mean dark neuron volume density in each unit of the tissue was assessed using stereological formulas. The obtained data were analyzed by Aanlysis of variance (ANOVA) in SPSS.<br>
<strong>Results:</strong> Compared with the control group, the average number of dark neurons in the hippocampus significantly increased following the administration of D-galactose (P=0.001). The average dark neurons frequency in the hippocampus significantly decreased in the 50 and 100 mg/kg curcumin-treated groups (P=0.001 for both) compared to that of the D-galactose treated animals.<br>
<strong>Conclusion:</strong> The results of this study showed that treatment with 100 mg/kg of curcumin reduced the number of dark neurons in the hippocampus of the aging mice. It seems that curcumin decreases dark neurons via the reduction of apoptosis. Also, curcumin is a powerful antioxidant and affects the level of antioxidant status in the brain.</div>
Ageing, Hippocampus, Neuron
63
68
http://pbr.mazums.ac.ir/browse.php?a_code=A-10-562-2&slc_lang=en&sid=1
Zahra
Heidari
HeidariZ94@mums.ac.ir
10031947532846005099
10031947532846005099
No
Department of Anatomy and Cell Biology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Shabnam
Mohammadi
mohammadish@mums.ac.ir
10031947532846005100
10031947532846005100
Yes
Department of Anatomy and Cell Biology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Mahdieh
Yousefi Taba
yousefiT941@mums.ac.ir
10031947532846005101
10031947532846005101
No
Department of Anatomy and Cell Biology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.