en فارماکوگنوزی Pharmacognosy پژوهشي Original Research Free radical production from different biological and environmental sources is due to an imbalance of natural antioxidants, which further leads to inflammation. Antioxidant metabolites are often characterized by anti-inflammatory and anticoagulation activity. Mangrove plants synthesize different classes of metabolites, including antioxidants, to minimize the devastating effect of oxidation resulting from the elevated salinity, UV, and other unique geochemical components. Accordingly, this study aimed at investigating the antioxidant, anti-inflammatory, and anticoagulation properties, as well as polyphenol content of the two selected mangrove plant species: <em>Aegiceras corniculatum</em> and <em>Acanthus ilicifolius</em>. We used the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, reducing power, ferric reducing antioxidant power (FRAP) assay, &beta;-carotene-linoleic acid bleaching assay (BCB), total phenolic content (TPC), total flavonoid&nbsp; content (TFC) and total tannin content (TTC) to determine antioxidant activity of the ethanol extract of <em>A. corniculatum</em> bark and leaves and <em>A. ilicifolius</em> leaves. Furthermore, human red blood cell (HRBC) membrane stabilization assay, lipoxygenase (LOX) inhibition assay, and prothrombin time (PT) test were performed for determining anti-inflammatory activity of the samples. <em>A. corniculatum</em> bark is a potent antioxidant (IC₅₀ 20.49 &plusmn; 2.14 &micro;g/mL in DPPH assay) with anti-inflammatory (IC₅₀ 23.58 &plusmn; 1.75 &micro;g/mL in LOX inhibition assay) and anticoagulation activity (18.19 &plusmn; 0.13 min in prothrombin time assay) compared to other extracts. All extracts were found with significant (<em>P</em><0.001) antioxidant, anti-inflammatory, and anticoagulation properties. Further studies on liquid chromatography-mass spectrometry (LC-MS) analysis, anti-inflammatory, and anticoagulation are recommended. Antioxidant, Anti-inflammatory, Anticoagulation, Mangrove, Sundarbans 35 44 http://pbr.mazums.ac.ir/browse.php?a_code=A-10-726-1&slc_lang=en&sid=1 Rana Biswas tusharkb07@outlook.com 10031947532846004020 10031947532846004020 Yes Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna-9208, Bangladesh S. M. Mahbubur Rahman 10031947532846004021 10031947532846004021 No Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna-9208, Bangladesh Kazi Mohammed Didarul Islam 10031947532846004022 10031947532846004022 No Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna-9208, Bangladesh Md. Morsaline Billah 10031947532846004023 10031947532846004023 No Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna-9208, Bangladesh Afiya Aunjum 10031947532846004024 10031947532846004024 No Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna-9208, Bangladesh Tauhidur Rahman Nurunnabi 10031947532846004025 10031947532846004025 No Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna-9208, Bangladesh Sujan Kumar Kundu 10031947532846004026 10031947532846004026 No Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna-9208, Bangladesh Md. Emdadul Islam 10031947532846004027 10031947532846004027 No Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna-9208, Bangladesh