Pharmaceutical and Biomedical Research
Pharm Biomed Res
Medical Sciences
http://pbr.mazums.ac.ir
1
admin
2423-4486
2423-4494
10.29252/pbr
8888
en
jalali
1398
6
1
gregorian
2019
9
1
5
3
online
1
fulltext
en
Chemical Composition and Bioactivity of the Essential Oil of Cassia singueana Flowers Growing in Nigeria
فارماکوگنوزی
Pharmacognosy
پژوهشي
Original Research
<em>Cassia singueana</em> (Delile) Lock from the family Fabaceae is a well-known medicinal plant that grows abundantly in Nigeria and other African countries, and has long been used in the treatment of various ailments including malaria and other infectious diseases. The present study aimed at assessing the composition, and bioactivity of the essential oil of the flowers of <em>C. singueana </em>collected from Nigeria. The essential oil was extracted by hydrodistillation and the chemical composition was analyzed by gas chromatography (GC) coupled with a flame ionization detector (GC-FID) and GC coupled to mass spectrometry (GC–MS). The bioactivity of the oil was determined using the brine shrimp lethality assay, agar diffusion antimicrobial test, the 2, 2-diphenylpicrylhydrazyl, metal chelation, and superoxide anion antioxidant assays. The essential oil yield and the percentage of identified compounds were 1.58% and 97.91%, respectively. More than 20 compounds were identified. The major component was geranyl acetone (36.82%) followed by phytol (18.12%). The essential oil showed lethality against the brine shrimp larvae with an LC<sub>50</sub> value of 18.7 µg/ml, and antimicrobial activity with largest inhibition zones of 32-33 mm against <em>Candida albicans, Streptococcus pneumoniae,</em> and <em>Staphylococcus aureus</em>. The oil also exhibited considerable antioxidant activity as evident from its ability to scavenge free-radicals such as DPPH, superoxide anions, and metal-chelation.
Cassia singueana, Fabaceae,Essential oils, Antimicrobial, Antioxidant, Brine shrimp lethality assay
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7
http://pbr.mazums.ac.ir/browse.php?a_code=A-10-680-1&slc_lang=en&sid=1
Bilkisu
Adedoyin
10031947532846003991
10031947532846003991
Yes
Department of Pure and Applied Chemistry, Faculty of Science, Usman Danfodiyo University, Sokoto, Nigeria
Aminu
Muhammed
10031947532846003992
10031947532846003992
No
Department of Pure and Applied Chemistry, Faculty of Science, Usman Danfodiyo University, Sokoto, Nigeria
Sani
Mohammed Dangoggo
10031947532846003993
10031947532846003993
No
Department of Pure and Applied Chemistry, Faculty of Science, Usman Danfodiyo University, Sokoto, Nigeria
Abdullahi
Rabah
10031947532846003994
10031947532846003994
No
Centre for Natural Products Discovery, School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, James Parsons Building, Byrom Street, Liverpool, United Kingdom
George
Sharples
10031947532846003995
10031947532846003995
No
Centre for Natural Products Discovery, School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, James Parsons Building, Byrom Street, Liverpool, United Kingdom
Lutfun
Nahar
10031947532846003996
10031947532846003996
No
Centre for Natural Products Discovery, School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, James Parsons Building, Byrom Street, Liverpool, United Kingdom
Satyajit
Sarker
10031947532846003997
10031947532846003997
No
Centre for Natural Products Discovery, School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, James Parsons Building, Byrom Street, Liverpool, United Kingdom