en نانوتکنولوژی Nanotechnology پژوهشي Original Research Indonesia has various beneficial plant species which have not been cultivated well yet. Blimbi (<em>Averrhoa bilimbi</em> L.) is among those plants which have antibacterial activities against<em> Staphylococcus aureus</em> and <em>Escherichia coli</em>. As carriers, the nanoparticles of this plant dissolve, trap, encapsulate, and attach the chemical preparation inside its matrix. This study aimed to compare the effect of two forms of the nanoparticles using Blimbi extract (i.e., single state and ointment preparation) on the antibacterial activity against bacteria. In this experimental study, the Blimbi extract changed to nanoparticles followed by a drying process using a drier spray. Afterwards, the nanoparticles were tested for antibacterial activity and mixed with an ointment base. The Blimbi extract was formulated in a form of absorbent ointment preparation with dark brown color and unique fragrance with pH of 6.42-6.80 and spread ability of 6.16-6.90 mm. According to the results, the diameters of the inhibition zone of nanoparticles using Blimbi extract on <em>S. aureus</em> and <em>E. coli</em> were 20 and 19.75 mm, respectively. The nanoparticles levels were higher in <em>E. coli</em> (12.25 mm) than those in <em>S. aureus</em> (15.50 mm). Meanwhile, the nanoparticles levels in ointment preparation were 15.84 and 14.73 mm for <em>S. aureus</em> and <em>E. coli.</em> The nanoparticles using Blimbi extract and the ointment using Blimbi extract were safe and did not irritate the skin. Antibacterial, nanoparticle, formulation, Blimbi (Averrhoa bilimbi L.), ointment 32 39 http://pbr.mazums.ac.ir/browse.php?a_code=A-10-532-1&slc_lang=en&sid=1 Nia Lisnawati Nia Lisnawati lisnawatinia12@gmail.com 10031947532846003688 10031947532846003688 Yes Department of Pharmacy, IKIFA Pharmacy Academy, East Jakarta, Indonesia IKiFA Pharmacy Academy Shirly Kumala Shirly Kusuma lisnawatinia12@gmail.com 10031947532846003689 10031947532846003689 No Department of Pharmacy, Pancasila University, South Jakarta, Indonesia Pancasila University Deni Rahmat Deni Rahmat lisnawatinia12@gmail.com 10031947532846003690 10031947532846003690 No Department of Pharmacy, Pancasila University, South Jakarta, Indonesia Pancasila University