en فارماکولوژی Pharmacology پژوهشي Original Research Several human diseases including, cancer, rheumatoid arthritis, neurodegenerative and hepatic diseases are related to destructive effect of reactive oxygen species (ROS). Antioxidants may provide a possible solution to this problem.This study was carried out to investigate the effect of hydroalcoholic extract of <em>Celtis australis</em> on CCl₄ induced hepatotoxicity in mice.<br> &nbsp;The antioxidant activity of <em>C. australis</em> was evaluated by 2,2-diphenyl-1-picryl hydrazyl (DPPH) radical scavenging assay. For evaluation of hepatoprotective activity of extract the animals were pretreated with 200, and 400mg/kg b.w, intraperitoneally of <em>C. australis </em>extract for 7 days and then received CCl₄ (0.5 ml/kg b.w, in olive oil). Liver injury was determined by serum biochemical parameters such as Alanine aminotransferase (ALT), Aspartate aminotransferase (AST), and Alkaline phosphatase (ALP) and glutathione contents of liver tissue and histopathological studies. A significant reduction in the serum biochemical parameters was observed when compared to CCl₄ received group(p<0.001). The standard antioxidant used in the study was Ascorbic acid. <em>C. australis</em> extract significantly suppressed the increase in plasma activities of liver enzymes and effectively protected animals against CCl₄-induced hepatic tissue damages. This study confirmed the hepatoprotective effect of the hydroalcholic extract of <em>C. australis.</em> Celtis australis, CCl4, Liver, Antioxidant, Toxicity 26 31 http://pbr.mazums.ac.ir/browse.php?a_code=A-10-452-1&slc_lang=en&sid=1 mohammad shokrzadeh mslamouk@yahoo.com 10031947532846003683 10031947532846003683 Yes Department of Toxicology and Pharmacology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran hossein bakhshi jouybari ho3einbakhshi@yahoo.com 10031947532846003684 10031947532846003684 No Department of Pharmacognosy and Biotechnology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran Majedeh hosseinpour mhosseinpour@yahoo.com 10031947532846003685 10031947532846003685 No Student Research Committee, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran ali ziar Aziar@yahoo.com 10031947532846003686 10031947532846003686 No Student Research Committee, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran emran habibi emrapharm@yahoo.com 10031947532846003687 10031947532846003687 No Pharmaceutical Sciences Research Center, Mazandaran University of Medical Sciences, Sari, Iran