en داروسازی صنعتی Industrial Pharmacy پژوهشي Original Research <p>Tolterodine tartrate, is a new, potent and competitive muscarinic receptor antagonist in clinical development for the treatment of urge incontinence and other symptoms of unstable bladder. The purpose of this study is to establish a reliable and quick method for the assignment of tolterodine tartrate by high performance liquid chromatography with ultraviolet detection (HPLC-UV). A rapid&nbsp; and&nbsp; sensitive&nbsp; high&nbsp; performance&nbsp; liquid&nbsp; chromatographic&nbsp; (HPLC)&nbsp; method&nbsp; has&nbsp; been developed&nbsp; for&nbsp; determination&nbsp; of&nbsp; tolterodine tartrate.&nbsp; Mobile phase was composed of phosphate acetate 0.1 M (pH 2.5)-acetonitrile (50:50 v/v) with a flow rate of 1.2 ml/min. The eluted peaks were detected by a UV detector was set at wavelength of 285 nm. The method was validated in the range of tolterodine tartrate concentrations from 10 to 100 &micro;g/ml. The limits of detection (LOD) and quantitation (LOQ) of the method were 5 and 10 &micro;g/ml, respectively. The average drug recovery was 98.20 % throughout the linear concentration range. The average within-run and between-run accuracy values of 98.56 % and 99.11 % respectively. Statistical&nbsp; assessment&nbsp; of&nbsp; various&nbsp; <em>in&nbsp; vitro</em>&nbsp; dissolution&nbsp; parameters&nbsp; and&nbsp; assay&nbsp; results was&nbsp; also&nbsp; conducted&nbsp; to&nbsp; establish&nbsp; if&nbsp; there were&nbsp; any significant difference among them. The validated HPLC method has been used successfully to study tolterodine tartrate.</p> Tolterodine tartrate, HPLC, assay, dissolution 47 57 http://pbr.mazums.ac.ir/browse.php?a_code=A-10-47-9&slc_lang=en&sid=1 Hossein Danafar 1003194753284600974 1003194753284600974 Yes Zanjan Pharmaceutical Nanotechnology Research Center, Zanjan University of Medical Sciences, Zanjan, Iran