en آنالیز دارو Drug Analysis پژوهشي Original Research <p>A simple and sensitive high performance liquid chromatography-electrospray ionization mass spectrometry method has been evaluated for the assignment of clonidine hydrochloride in human plasma. The mobile phase composed of acetonitrile&ndash;water 60:40 (v/v), and 0.2% formic acid 20 &micro;L of sample was chromatographically analyzed using a repacked ZORBAX-XDB-ODS C₁₈ column (2.1 mm&times;30 mm, 3.5 micron). Detection of analytes was achieved by tandem mass spectrometry with electrospray ionization (ESI) interface in positive ion mode was operated under the multiple-reaction monitoring mode (m/z 230.0 &rarr; 213). Sample pretreatment involved in a one-step protein precipitation (PPT) with methanol and percholoric acid (HClO₄) of 0.15 mL plasma. Standard curve was linear (<em>r </em>= 0.998) over the concentration range of 0.01-10.0 ng/ml and showed suitable accuracy and precision. The limit of quantification (LOQ) was 0.01 ng/ml. The method is rapid, simple, very steady and precise for the separation, assignment, evaluation of clonidine healthy in human plasma.</p> Clonidine hydrochloride, LC-MS, Human plasma 48 58 http://pbr.mazums.ac.ir/browse.php?a_code=A-10-47-6&slc_lang=en&sid=1 Hossein Danafar 10031947532846001082 10031947532846001082 Yes Zanjan Pharmaceutical Nanotechnology Research Center, Zanjan University of Medical Sciences, Zanjan, Iran