en بیولوژی سلولی- مولکولی Cellular and Molecular Biology پژوهشي Original Research <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:10%"><span style="unicode-bidi:embed"><span style="line-height:200%"><span new="" roman="" style="font-family:" times=""><b><span style="color:black">Background</span></b><span style="color:black">: Ovarian Cancer metastasis is the leading cause of cancer-related deaths in women worldwide. The effects of Quillaja saponaria bark saponin (QBS) on the viability and epithelial&ndash;mesenchymal transition (EMT) potential of human epithelial ovarian SKOV-3 and A2780 cells, and its anti-metastatic and anti-cancer efficacy of QBS </span><span lang="EN" style="color:black">in ovarian cancer model of rat have been investigated</span><span style="color:black">.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:10%"><span style="unicode-bidi:embed"><span style="line-height:200%"><span new="" roman="" style="font-family:" times=""><b><span style="color:black">Methods</span></b><span style="color:black">: The cell cytotoxicity of QBS against SKOV3 and A2780 cells evaluated by MTT assay to calculate IC50 values. </span><span lang="EN" style="color:black">Cell migration and invasion </span><span style="color:black">assessed by scratch assay. To explore metastasis molecular changes, the expression of key epithelial mesenchymal transition (EMT)-related genes, including <i>&alpha;5-integrin</i>, <i>MMP2</i>, <i>Zeb1</i>, <i>E-cadherin</i></span> <span style="color:black">measured by real-time PCR. For <i>in vivo</i> assay, DMBA-induced ovarian cancer rat model used.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:10%"><span style="unicode-bidi:embed"><span style="line-height:200%"><span new="" roman="" style="font-family:" times=""><b><span lang="EN" style="color:black">Results</span></b><span lang="EN" style="color:black">: </span><span style="color:black">MTT assay demonstrated that QBS didn&rsquo;t exert significant toxicity on </span>NIH3T3 as normal cells, nevertheless <span style="color:black">induced cytotoxicity against A2780 and SKOV3 cells in a dose and time-dependent manner, IC50 was determined as 82 and 77 &mu;g/ml for SKOV3 and A2780 cells, respectively. The scratch assay revealed that QBS significantly attenuated cell invasion at suc-IC50 concentrations (20 and 30 &micro;g/ml). Gene expression indicated down-regulation of <i>Zeb1</i>, <i>&alpha;5-integrin</i> and <i>MMP2</i> at IC50 value of both SKOV3 and A2780 cells (p &le; 0.001). Also, up-regulation of <i>E-cadherin</i> for both cell lines at IC50 value were significant (p &le; 0.001). <i>In vivo</i> assay exhibited that QBS significantly reduced tumor growth (p&le;0.01) and protected rats against DMBA-induced ovarian cancer with an optimal effect at the dose of 16 mg/kg.</span> <span style="color:black"></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:10%"><span style="unicode-bidi:embed"><span style="line-height:200%"><span new="" roman="" style="font-family:" times=""><b><span lang="EN" style="color:black">Conclusion</span></b><span lang="EN" style="color:black">: </span><span style="color:black">Overall, QBS can offer as potent anti-tumoral agent against ovarian cancer cells <i>in vitro </i>and <i>in vivo</i>.</span><span lang="EN" style="color:black"></span></span></span></span></span></span></span><br> &nbsp; Ovarian cancer metastasis, Quillaja saponaria bark saponin, α5-integrin, MMP2, Zeb1, E-cadherin. 0 0 http://pbr.mazums.ac.ir/browse.php?a_code=A-10-1469-2&slc_lang=en&sid=1 Raoufeh Koochaki Raoufeh.koochaki73@gmail.com 100319475328460012375 100319475328460012375 No Department of Cell & Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran. Mohammad Nabiuni nabiuni@khu.ac.ir 100319475328460012376 100319475328460012376 Yes Department of Cell & Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran. Elaheh Amini elaheh.amini@khu.ac.ir 100319475328460012377 100319475328460012377 No Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran.