Pharmaceutical and Biomedical Research
Pharm Biomed Res
Medical Sciences
http://pbr.mazums.ac.ir
1
admin
2423-4486
2423-4494
10.29252/pbr
8888
en
jalali
1394
6
1
gregorian
2015
9
1
1
3
online
1
fulltext
en
Hydroalcoholic and polyphenolic extracts of Ziziphus Jujuba Mill fruits prevent methyl methanesulfonate-induced DNA damage in HepG2 cells
ترکیبات طبیعی
Natural products
پژوهشي
Original Research
<p><em>Ziziphus jujuba Mill </em>(ZJ), which has been extensively used by the Iranian traditional healers, belongs to the <em>Ramnaceae </em>family. This semitropical herb contains large quantities of polyphenols and flavonoids, which in turn reveal antioxidant, antibacterial, free radical scavengering, and several other pharmacological activities. The purpose of the present study was to evaluate the DNA damage prevention potential of hydroalcoholic and polyphenolic extracts of <em>Ziziphus jujuba</em> on HepG2 cells. Throughout the assessment of genoprotective properties, cells were incubated with various concentrations of hydroalcoholic (0.1, 1, 10, and 50 µg/ml) and polyphenolic extracts (0.1 and 1 µg/ml) for a one-hour period, followed by a one-hour incubation period with genotoxic concentration of methyl methanesulfonate (MMS) (10 µM). The comet assay method was applied because of its being attributable to the substantial sensitivity, its inexpensiveness, and its straightforward procedure of use. The tail length, percentage of DNA in tail, and tail moment were measured. Statistical analysis revealed that concentrations of 10 μg/ml for hydroalcoholic extract and 1μg/ml for polyphenolic extract were genoprotective against MMS. Therefore, our results suggest that <em>Ziziphus jujuba</em> at suitable doses can prevent DNA damage.</p>
Ziziphus Jujuba, Genoprotective effect, HepG2, Comet Assay.
20
30
http://pbr.mazums.ac.ir/browse.php?a_code=A-10-26-17&slc_lang=en&sid=1
Mahmoud
Etebari
10031947532846001106
10031947532846001106
Yes
Department of Pharmacology and Toxicology, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.
Behzad
Zolfaghari
10031947532846001107
10031947532846001107
No
Department of Pharmacognosy, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
Abbas
Jafarian-Dehkordi
10031947532846001108
10031947532846001108
No
Department of Pharmacology and Toxicology, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.
Afsaneh
Mirzaei
10031947532846001109
10031947532846001109
No
Department of Pharmacology and Toxicology, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.