Pharmaceutical and Biomedical Research
Pharm Biomed Res
Medical Sciences
http://pbr.mazums.ac.ir
1
admin
2423-4486
2423-4494
10.29252/pbr
8888
en
jalali
1400
7
1
gregorian
2021
10
1
7
4
online
1
fulltext
en
The Effect of Honey Bee Venom on CD14 Protein Expression as Monocyte Marker in D-Alpha-Tocopheryl Succinate (Vitamin E)-Treated HL-60 Cells
بیولوژی سلولی- مولکولی
Cellular and Molecular Biology
پژوهشي
Original Research
<strong>Background</strong>: The membrane form of a cluster of differentiation 14 (CD14) is anchored to the phospholipid bilayer via glycosylphosphatidylinositol anchor. This molecule is expressed on the intrinsic surface of monocytes and neutrophils. This protein is not expressed on the HL-60 cell surface. It is believed that the differentiation of HL-60 cells stops in the promyelocytic stage. The differentiation of HL-60 cells with compounds such as vitamin A, D, E results in membrane CD14 expression.<br>
<strong>Objectives</strong>: The objective of this study was an evaluation of CD14 expression by Honey Bee Venom (HBV) in HL60 cells treated by D-alpha-tocopheryl succinate (D-α-TS).<br>
Methods: HL-60 cells were cultured in RPMI Media 1640 medium and treated with different concentrations of D-α-TS and HBV. Cellular differentiation was tested by nitro blue tetrazolium (NBT) staining, immunocytochemistry, and flow cytometry. The studied data were analyzed using one-way analysis of variance and InStat 3 software.<br>
<strong>Methods</strong>: HL-60 cells were cultured in RPMI medium and treated with different concentrations of D-α-TS and HBV. Cellular differentiation was tested by NBT staining, immunocytochemistry, and flow cytometry. The studied data were analyzed using one-way analysis of variance and InStat 3 software.<br>
<strong>Results</strong>: MTT assay demonstrated that HBV and D-alpha-tocopheryl induce death in HL-60 cell lines in a time and dose-dependent manner. Also, Wright-Giemsa, NBT staining showed morphologically differentiation. Immunocytochemistry and flow cytometry analysis shows that cells treated with 6 µg/mL D-α-TS and 2.5 µg/mL HBV for 5 days significantly increase the expression of CD14 in HL-60 compared to cells treated with D-α-TS.<br>
<strong>Conclusion</strong>: HBV can induce cell death and inhibit cell proliferation. Also, increase differentiation potency of D-α-TS via HBV can increase the differentiation by inhibiting NF-κB and COX-2 and increasing the expression of P21 that plays an essential role in increasing CD14 protein expression and subsequently induce differentiation in HL-60 cells to monocytes.<br>
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Differentiation, Hematopoietic stem cell, Promyelocytic leukemia, p21 protein
261
270
http://pbr.mazums.ac.ir/browse.php?a_code=A-10-802-1&slc_lang=en&sid=1
Azam
Yarahmadi
yarahmadi_azam@yahoo.com
10031947532846007638
10031947532846007638
No
Department of Animal Biology, School of Biological Sciences, Kharazmi University, Tehran, Iran.
Mohammad
Nabiuni
devbiokharazmi@gmail.com
10031947532846007639
10031947532846007639
No
Department of Cell and Molecular Biology, School of Biological Sciences, Kharazmi University, Tehran, Iran.
Latifeh
Karimzadeh Bardei
latifehkarimzadeh@gmail.com
10031947532846007640
10031947532846007640
No
Department of Biology, School of Science, University of Tehran, Tehran, Iran.
Majid
Kabuli
mkabuli@sina.tums.ac.ir
10031947532846007641
10031947532846007641
Yes
Department of Medical Genetics, School of Medicine, Tehran University of Medical Science, Tehran, Iran.