Pharmaceutical and Biomedical Research
Pharm Biomed Res
Medical Sciences
http://pbr.mazums.ac.ir
1
admin
2423-4486
2423-4494
10.29252/pbr
8888
en
jalali
1399
9
1
gregorian
2020
12
1
6
1
online
1
fulltext
en
The Effect of Exercise, Ozone, and Mesenchymal Stem Cells Therapy on CB-1 and GABA Gene Expression in the Cartilage Tissue of Rats With Knee Osteoarthritis
عمومی
General
پژوهشي
Original Research
<div style="text-align: justify;"><strong>Background</strong>: Studies have reported the beneficial effects of exercise, ozone therapy, and stem cell therapy for the treatment of knee osteoarthritis. <br>
<strong>Objectives</strong>: To reduce the duration of the treatment, we decided to investigate the synergistic effects of Endurance Training (ET), ozone therapy, and mesenchymal stem cell (MSCs) therapy separately or in combination on the cannabinoid receptors 1 (CB-1) and Gamma-Aminobutyric Acid (GABA) gene expression, as anti-nociceptive pathways in the cartilage tissue of rats with osteoarthritis. <br>
<strong>Methods</strong>: In this experimental study, 40 rats with knee osteoarthritis were divided into eight groups of 5 rats each: 1. Osteoarthritis; 2. MSCs; 3. Ozone therapy; 4. ET; 5. ozone therapy+MSCs, 6. ET+ozone therapy; 7. ET+MSCs, and 8. ET+MSCs+ozone therapy. Knee osteoarthritis was induced through the partial cutting of internal meniscus. The endurance training program was initiated with a 30-min run on a 0-degree slope treadmill at a speed of 16 m/min in the first week that gradually reached to 50 minutes after the 8 weeks. Rats in the MSCs groups received an intra-articular injection of 1×106 cells/kg into the right knee joints. Also, ozone therapy groups were injected at a concentration of 20 μg/mL once a week for three weeks. Rats were then anesthetized 48 h after their last treatment. The Independent t test and 3-way ANOVA were used to analyze the findings (P≤0.05). <br>
<strong>Results</strong>: ET, ozone therapy, and MSCs alone increased CB-1 and GABA gene expression in the cartilage tissue of rats with knee osteoarthritis (P≤0.05 for all). A combination of ozone therapy and ET (P≤0.05) and combination of ozone therapy and MSCs (P≤0.05) were significant in increasing CB-1 and GABA gene expression. However, the combination of ET and MSCs (P≥0.05) and combination of ET, MSCs, and ozone therapy (P≤0.05) significantly increased CB-1 gene expression. <br>
<strong>Conclusion</strong>: It seems that ET, ozone therapy, and MSCs alone can have favorable effects on CB-1 and GABA variables. Also, the combination of ET and ozone therapy and also ozone therapy and MSCs have favorable effects on the anti-nociceptive pathway in the animal model of osteoarthritis. But further studies are needed on the combination of ET and MSCs and also the combination of ET, ozone therapy, and MSCs.</div>
Osteoarthritis, Mesenchymal stem cells, Ozone, Training, Anti-nociceptive
45
52
http://pbr.mazums.ac.ir/browse.php?a_code=A-10-767-1&slc_lang=en&sid=1
Maryam
Rezaie
maryam.rezaei3320@gmail.com
10031947532846005123
10031947532846005123
Yes
Department of Sport Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
Mohammad Ali
Azarbayjani
ali.azarbayjani@gmail.com
10031947532846005124
10031947532846005124
No
Department of Sport Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
Maghsoud
Peeri
m.peeri@gmail.com
10031947532846005125
10031947532846005125
No
Department of Sport Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
Seyed Ali
Hosseini
alihoseini_57@yahoo.com
10031947532846005126
10031947532846005126
No
Department of Sport Physiology, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran.