Pharmaceutical and Biomedical Research
Pharm Biomed Res
Medical Sciences
http://pbr.mazums.ac.ir
1
admin
2423-4486
2423-4494
10.29252/pbr
8888
en
jalali
1398
1
1
gregorian
2019
4
1
5
2
online
1
fulltext
en
Edible films based on chitosan-flaxseed mucilage: in vitro antimicrobial and antioxidant properties and their application on survival of food-borne pathogenic bacteria in raw minced trout fillets
عمومی
General
پژوهشي
Original Research
<div dir="rtl" style="text-align: left;">Various natural oils/extracts and their constituents incorporated into biopolymer-based edible films as a promising technology with the knowledge that these compounds have been able to reduce microbial growth and chemical changes of packed foodstuffs. The objective of this study was to evaluate the effect of incorporation of <em>Ziziphora clinopodioides </em>essential oil (ZEO; 0, 0.25 and 0.5%) and sesame oil (SO; 0, 0.5 and 0.75%) into chitosan-flaxseed mucilage (CH-FM) film against <em>Listeria monocytogenes</em>, <em>Salmonella typhimurium</em>, <em>Staphylococcus aureus</em> and <em>Escherichia coli</em> O157:H7<em> in vitro</em> condition and raw minced trout fillets during refrigerated condition. The <em>in vitro</em> antibacterial and antioxidant properties of CH-FM films were evaluated using agar disk diffusion method and free radical scavenging activity assay, respectively. The most important constituents of ZEO were found to be carvacrol (65.22%), thymol (19.51%), <em>ɣ</em>-terpinene (4.63%) and <em>p-</em>cymene (4.86%). The lowest and highest antimicrobial effect against <em>S. aureus</em>, <em>L. monocytogenes</em>, <em>E. coli</em> O157:H7 and <em>S. typhimurium</em> were found for CH-FM films enriched with SO 0.5% (0.98-1.24 mm) and ZEO 0.5% + SO 0.75% (5.01-6.25 mm), respectively. The antioxidant property of CH-FM based films were found to be ranged 5.45% ± 0.04-37% ± 0.45. In treated trout fillets, the counts of <em>L. monocytogenes</em>, <em>S. aureus</em>,<em> E. coli</em> O157:H7 and <em>S. typhimurium </em>were 1.54-4.18, 0.34-3.35, 0.29-1.45 and 0.19-1.27 log CFU/g significantly lower than control groups after two weeks of refrigerated storage, respectively. The designated films had good antibacterial effect against some food borne pathogenic bacteria including <em>L. monocytogenes</em>, <em>S. aureus</em>,<em> S. typhimurium</em> and <em>E. coli</em> O157:H7 in raw rainbow trout fillets.</div>
Chitosan, flaxseed mucilage, Ziziphora linopodioides essential oil, sesame oil, rainbow trout fillet
10
16
http://pbr.mazums.ac.ir/browse.php?a_code=A-10-645-1&slc_lang=en&sid=1
Negin
Karami
نگین
کرمی
neginkarami87@gmail.com
10031947532846006182
10031947532846006182
No
Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, University of Tehran
گروه بهداشت و کنترل کیفی مواد غذایی دانشکده دامپزشکی دانشگاه تهران
Abolfazl
Kamkar
ابولفضل
کامکار
a.kamkar@ut.ac.ir
10031947532846006183
10031947532846006183
No
Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, University of Tehran
گروه بهداشت و کنترل کیفی مواد غذایی دانشکده دامپزشکی دانشگاه تهران
Yasser
Shahbazi
یاسر
شهبازی
y.shahbazi@razi.ac.ir
10031947532846006184
10031947532846006184
Yes
Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Razi University
گروه بهداشت و کنترل کیفی مواد غذایی دانشکده دامپزشکی دانشگاه رازی
Ali
Misaghi
علی
میثاقی
a.misaghi@ut.ac.ir
10031947532846006185
10031947532846006185
No
Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, University of Tehran
گروه بهداشت و کنترل کیفی مواد غذایی دانشکده دامپزشکی دانشگاه تهران