en فارماکوگنوزی Pharmacognosy پژوهشي Original Research <em>Arnebia euchroma</em> is from Boraginaceae family. Various therapeutic effects have been reported for different species of this plant. The aim of this study was to investigate antioxidant activity of hydroalcoholic extract of the root of <em>A. euchroma</em> and its protective effect on hepatotoxicity induced by CCl₄ in mice. In this experimental study, <em>A. euchroma</em> was extracted with ethanol solvent by maceration method. Antioxidant activity of the plant has been evaluated by the DPPH free radical scavenging method. Then, the hepatoprotective effect of <em>A. euchroma</em> was evaluated through determination the content of glutathione in liver tissue, ALP, AST, ALT serum levels and histopathology of liver in different groups. The total phenolic content determined as 0.56% per 100 g of plant was equivalent to gallic acid. The total flavonoid content was 0.079% per 100 g of plant that was equivalent to quercetin. The IC₅₀ of the extract for free radical scavenging was obtained 139.2 &micro;g/ml. The extract increased liver glutathione and serums ALP, AST, ALT that were decreased by CCl₄ administration in animal. All protective effects were dose-dependent and the results of liver histopathology showed a significant improvement of inflammation and necrosis that were caused by CCl₄ in mice. The results of this study revealed that the hydroalcoholic extract of <em>A. euchroma</em> root has an antioxidant effect and excellent hepatoprotection in mice. Hepatoprotective, arnebia euchroma, CCl4, mice 23 29 http://pbr.mazums.ac.ir/browse.php?a_code=A-10-55-2&slc_lang=en&sid=1 Mohammad Shokrzadeh 10031947532846003835 10031947532846003835 No Department of Pharmacology and Toxicology, Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran Mohammad Azadbakht محمد آزادبخت azadbakhtm@hotmail.com 10031947532846003836 10031947532846003836 Yes Department of Pharmacognosy, Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran مرکز تحقیقات علوم دارویی Hamidreza Shakibamanesh 10031947532846003837 10031947532846003837 No Student Research Committee Pharmacy, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran