Pharmaceutical and Biomedical Research
Pharm Biomed Res
Medical Sciences
http://pbr.mazums.ac.ir
1
admin
2423-4486
2423-4494
10.29252/pbr
8888
en
jalali
1396
2
1
gregorian
2017
5
1
3
1
online
1
fulltext
en
PLGA-based macrophage-mediated drug targeting for the treatment of visceral leishmaniasis
فارماسیوتیکس
Pharmaceutics
پژوهشي
Original Research
<p>The potential of PLGA-nanoparticles as a carrier of amphotericin B and doxorubicin against visceral leishmaniasis was evaluated by macrophage-mediated drug targeting approach. PLGA-nanoparticles were modified by coating them with macrophage-specific ligand-lectin. Prior to <em>in-vitro</em> studies, characterization studies were carried out systematically include particle size, surface morphology, percent drug entrapment and percent drug release. <em>In vitro</em> studies were carried out in J774.1 in order to evaluate the effective endocytotic uptake of nanoparticles by macrophages. The antileishmanial activity of PLGA-nanoparticles and lectin-PLGA-nanoparticles was tested <em>in-vitro</em> in <em>leishmania donovani</em> infected macrophage-amastigote system (J774A.1 cells), which showed higher efficacy of lectin grafted PLGA-nanoparticles over plain PLGA-nanoparticles. The prepared plain and lectin grafted PLGA-Nanoparticles based systems showed excellent potential for passive and active intra-macrophage targeting, respectively and the approach could be an effective alternative to the currently available drug regimens against VL.</p>
Visceral leishmaniasis, macrophage targeting, nanoparticles, amphotericin B, doxorubicin, lectin
41
47
http://pbr.mazums.ac.ir/browse.php?a_code=A-10-100-2&slc_lang=en&sid=1
Prachi
sharma
prachisharmaamity@gmail.com
10031947532846003749
10031947532846003749
No
Apeejay Stya University, Gurgaon, India
Swati
Gupta
swatig25@gmail.com
10031947532846003750
10031947532846003750
Yes
Department of Pharmaceutics, B. S. Anangpuria Institute of Pharmacy