دوره 1، شماره 4 - ( 9-1394 )                   جلد 1 شماره 4 صفحات 48-58 | برگشت به فهرست نسخه ها



DOI: 10.18869/acadpub.pbr.1.4.48

XML Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Danafar H. Method validation of clonidine hydrochloride in human plasma by LC-MS technique . mazums-pbr. 2015; 1 (4) :48-58
URL: http://pbr.mazums.ac.ir/article-1-83-fa.html
Method validation of clonidine hydrochloride in human plasma by LC-MS technique . . 1394; 1 (4) :48-58

URL: http://pbr.mazums.ac.ir/article-1-83-fa.html


چکیده:   (1446 مشاهده)

A simple and sensitive high performance liquid chromatography-electrospray ionization mass spectrometry method has been evaluated for the assignment of clonidine hydrochloride in human plasma. The mobile phase composed of acetonitrile–water 60:40 (v/v), and 0.2% formic acid 20 µL of sample was chromatographically analyzed using a repacked ZORBAX-XDB-ODS C18 column (2.1 mm×30 mm, 3.5 micron). Detection of analytes was achieved by tandem mass spectrometry with electrospray ionization (ESI) interface in positive ion mode was operated under the multiple-reaction monitoring mode (m/z 230.0 → 213). Sample pretreatment involved in a one-step protein precipitation (PPT) with methanol and percholoric acid (HClO4) of 0.15 mL plasma. Standard curve was linear (r = 0.998) over the concentration range of 0.01-10.0 ng/ml and showed suitable accuracy and precision. The limit of quantification (LOQ) was 0.01 ng/ml. The method is rapid, simple, very steady and precise for the separation, assignment, evaluation of clonidine healthy in human plasma.

متن کامل [PDF 513 kb]   (726 دریافت)    
نوع مطالعه: پژوهشي | موضوع مقاله: آنالیز دارو

ارسال نظر درباره این مقاله : نام کاربری یا پست الکترونیک شما:
کد امنیتی را در کادر بنویسید

کلیه حقوق این وب سایت متعلق به می باشد.

طراحی و برنامه نویسی : یکتاوب افزار شرق

© 2015 All Rights Reserved | Pharmaceutical and Biomedical Research

Designed & Developed by : Yektaweb