Volume 10, Issue 4 (2024)
Pharm Biomed Res 2024, 10(4): 0-0 |
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Awatef E, mariem C, imen G, samira J, rim M, tahia B et al . Effects of Barium Chloride Graded Doses on Metallothioneins induction, Biochemical and Histomorphological Changes in the Kidney of Adult female rats. Pharm Biomed Res 2024; 10 (4)
URL: http://pbr.mazums.ac.ir/article-1-604-en.html
URL: http://pbr.mazums.ac.ir/article-1-604-en.html
Elwej Awatef * 
1, Chaabane Mariem1 , Ghobel Imen1 , Jebahi Samira2 , Marrekchi Rim3 , Boudawara Tahia4 , Zeghal Najiba5
1, Chaabane Mariem1 , Ghobel Imen1 , Jebahi Samira2 , Marrekchi Rim3 , Boudawara Tahia4 , Zeghal Najiba5
1- Animal Physiology Laboratory, UR11/ ES 70, Sciences Faculty of Sfax
2- National Center for Sciences and Nuclear Technologies, BiotechPole, Ariana 2020, Tunisia
3- Biochemistry Laboratory, CHU Hedi Chaker, University of Sfax
4- Anatomopathology Laboratory, CHU Habib Bourguiba, Sfax University
5- Animal Physiology Laboratory. Sfax Faculty of Sciences.BP1171, 3000 Sfax. Sfax University, Tunisia
2- National Center for Sciences and Nuclear Technologies, BiotechPole, Ariana 2020, Tunisia
3- Biochemistry Laboratory, CHU Hedi Chaker, University of Sfax
4- Anatomopathology Laboratory, CHU Habib Bourguiba, Sfax University
5- Animal Physiology Laboratory. Sfax Faculty of Sciences.BP1171, 3000 Sfax. Sfax University, Tunisia
Abstract: (65 Views)
Background: Heavy metals exposure causes usually harmful effects to the environment and human health.
Objective: The aim of the present study was to investigate the toxic effects of barium chloride (BaCl2) administered at graded doses to adult rats on kidney oxidative stress biomarkers and histological changes.
Methods: Animals were divided into four groups of six animals each: the control group, the group 2, 3 and 4 are exposed to barium at doses 67,150 and 300 ppm for 3 weeks. After recording 24 h urine volumes, samples were collected and then stored at –20 °C for further analysis. At the end of the experimental period, plasma and kidney tissue, were collected for biochemical and histological analysis.
Results: Our results revealed that treatment of barium induced nephrotoxicity as evidenced by a significant increase of malondialdehyde, hydrogen peroxide and advanced oxidation protein product. A significant increase in kidney levels of total metallothioneins was also observed. Superoxide dismutase, catalase, glutathione peroxidase activities, reduced glutathione, non protein thiol and vitamin C levels showed a significant decline. Plasma creatinine and urea levels showed a significant increase associated with a decrease in uric acid level. Nephro-biochemical parameters were substantiated by histological examination, which revealed disorganized kidney architecture.
Objective: The aim of the present study was to investigate the toxic effects of barium chloride (BaCl2) administered at graded doses to adult rats on kidney oxidative stress biomarkers and histological changes.
Methods: Animals were divided into four groups of six animals each: the control group, the group 2, 3 and 4 are exposed to barium at doses 67,150 and 300 ppm for 3 weeks. After recording 24 h urine volumes, samples were collected and then stored at –20 °C for further analysis. At the end of the experimental period, plasma and kidney tissue, were collected for biochemical and histological analysis.
Results: Our results revealed that treatment of barium induced nephrotoxicity as evidenced by a significant increase of malondialdehyde, hydrogen peroxide and advanced oxidation protein product. A significant increase in kidney levels of total metallothioneins was also observed. Superoxide dismutase, catalase, glutathione peroxidase activities, reduced glutathione, non protein thiol and vitamin C levels showed a significant decline. Plasma creatinine and urea levels showed a significant increase associated with a decrease in uric acid level. Nephro-biochemical parameters were substantiated by histological examination, which revealed disorganized kidney architecture.
Type of Study: Original Research |
Subject:
Toxicology
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