Volume 10, Issue 4 (2024)
Pharm Biomed Res 2024, 10(4): 303-318 |
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Shaik B, Swain B, Gupta Maddi E, Maddi S. Novel RP-UFLC Methodology for Concurrent Assessment of Curcumin, Resveratrol, Silybin, and Mangiferin From Topical Hydrogels. Pharm Biomed Res 2024; 10 (4) :303-318
URL: http://pbr.mazums.ac.ir/article-1-603-en.html
URL: http://pbr.mazums.ac.ir/article-1-603-en.html
1- Formulation and Analytical Research and Development Division, Acubiosys Private Limited, Hyderabad, India.
2- Department of Biopharmaceutics and Pharmaceutical Technology, Krupanidhi College of Pharmacy, Bangalore, India.
2- Department of Biopharmaceutics and Pharmaceutical Technology, Krupanidhi College of Pharmacy, Bangalore, India.
Abstract: (1126 Views)
Background: In topical preparations, four herbal compounds, namely mangiferin (MF),
resveratrol (RV), silybin (SB), and curcumin (CR) need to be quantified quickly and accurately.
Objective: This study utilizes a Franz diffusion cell setup to evaluate the suitability of reversed-phase ultra-fast liquid chromatography (UFLC) for assessing MF, RV, SB, and CR in a mouse skin.
Methods: A C-18 column (250×4.6 mm, 5 µm) was used at 40 °C for chromatographic separation. The mobile phase, a gradient of acetonitrile and 0.1% formic acid in high-performance liquid chromatography (HPLC) grade water was pumped at a rate of 1 mL per min. A 20 µL sample was loaded, and analytes were accessed using a photodiode array (PDA) detector. The method validation included selectivity, linearity (2-20 µg/mL), accuracy, precision, robustness, and sensitivity. The method’s run time was 20 min.
Results: The UFLC method was selective for the given compounds, and over the concentration range of 2–20 µg/mL, the method’s linearity was demonstrated with a correlation coefficient >0.99. Additionally, it was accurate, precise, robust, and sensitive, with a total run time of 20 min.
Conclusion: The validated reversed-phase UFLC method meets the highlighted need for accurate and quick measurement of MF, RV, SB, and CR in topical formulations. Its effective validation and application in researching drug features using a Franz diffusion cell apparatus make it an important analytical tool in pharmaceutical research, ensuring precise drug content, homogeneity, and release properties in topical formulations.
resveratrol (RV), silybin (SB), and curcumin (CR) need to be quantified quickly and accurately.
Objective: This study utilizes a Franz diffusion cell setup to evaluate the suitability of reversed-phase ultra-fast liquid chromatography (UFLC) for assessing MF, RV, SB, and CR in a mouse skin.
Methods: A C-18 column (250×4.6 mm, 5 µm) was used at 40 °C for chromatographic separation. The mobile phase, a gradient of acetonitrile and 0.1% formic acid in high-performance liquid chromatography (HPLC) grade water was pumped at a rate of 1 mL per min. A 20 µL sample was loaded, and analytes were accessed using a photodiode array (PDA) detector. The method validation included selectivity, linearity (2-20 µg/mL), accuracy, precision, robustness, and sensitivity. The method’s run time was 20 min.
Results: The UFLC method was selective for the given compounds, and over the concentration range of 2–20 µg/mL, the method’s linearity was demonstrated with a correlation coefficient >0.99. Additionally, it was accurate, precise, robust, and sensitive, with a total run time of 20 min.
Conclusion: The validated reversed-phase UFLC method meets the highlighted need for accurate and quick measurement of MF, RV, SB, and CR in topical formulations. Its effective validation and application in researching drug features using a Franz diffusion cell apparatus make it an important analytical tool in pharmaceutical research, ensuring precise drug content, homogeneity, and release properties in topical formulations.
Type of Study: Original Research |
Subject:
Industrial Pharmacy
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