en ترکیبات طبیعی Natural products پژوهشي Original Research <div style="text-align: justify;"><strong>Background</strong>: Saffron (Zaeferan in Persian), known as Red Gold, is obtained from the dried stigma of the Crocus sativus L. (Iridacea) plant. It plays a critical role in non-oil exporting in the economy of Iran, and the livelihoods of many farmers and workers depend on its yields. Burkholderia (Pseudomonas) gladioli pv. gladioli causes severe reductions the saffron yield in Iran. According to responsible authorities of Iran, no pesticides are registered or recommended for saffron cultivations (except for two herbicides). Most essential oils are listed as generally recognized as safe substances and can exhibit a crucial role in exporting pesticides free saffron.&nbsp;<br> Objectives: The study aimed to identify the most effective antibacterial essential oils against B. gladioli pv. gladioli.<br> <strong>Methods</strong>: Essential oils were extracted using a clevenger apparatus from 25 medicinal plants belonging to the Lamiaceae, Compositae, and Apiaceae families, followed by an initial evaluation through disk diffusion, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) assays. Dimethyl sulfoxide (DMSO) and amikacin served as a negative and positive control, respectively.<br> <strong>Results</strong>: Out of 25 essential oils, only 4 of them exhibited antibacterial effects on this pathogen including Satureja hortensis (22.66 mm inhibition), Thymus vulgaris (20.33 mm), Trachyspermum ammi (17.66 mm), and Sclerorhachis leptoclada (10.66 mm). Their MICs were 1, 2, 4, and 32 &micro;L/mL, respectively.&nbsp;<br> <strong>Conclusion</strong>: These findings highlight promising essential oils for controlling B. gladioli, supporting pesticide-free saffron production and safeguarding Iran&rsquo;s valuable crop yields.</div> Antibacterial activity, essential oils, Burkholderia gladioli, Crocus sativus L., Pesticide-free crop protection, Saffron 133 140 http://pbr.mazums.ac.ir/browse.php?a_code=A-10-1061-7&slc_lang=en&sid=1 Mahmoud Reza Karimi-shahri m.karimishahri@areeo.ac.ir; karimi4597@gmail.com 100319475328460012164 100319475328460012164 Yes Department of Plant Protection, Khorasan Razavi Agricultural & Natural Resources Research Center, Agricultural Research, Education and Extension Organization (AREEO), Mashhad, Iran. Samaneh Rahamouz-Haghighi rahamouz_haghighi.s@yahoo.com 100319475328460012165 100319475328460012165 No Department of Plant Protection, Khorasan Razavi Agricultural & Natural Resources Research Center, Agricultural Research, Education and Extension Organization (AREEO), Mashhad, Iran. Arash Honarmand arashhonarmand68@gmail.com 100319475328460012166 100319475328460012166 No Department of Plant Protection, Faculty of Agriculture, Azarbaijan Shahid Madani University, Tabriz, Iran. Elaheh Taheri taheri.elaheh1990@gmail.com 100319475328460012167 100319475328460012167 No Department of Plant Protection, Khorasan Razavi Agricultural & Natural Resources Research Center, Agricultural Research, Education and Extension Organization (AREEO), Mashhad, Iran.